This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, J.-K.
Right arrow Articles by Kapil, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, J.-K.
Right arrow Articles by Kapil, S.

 Previous Article  |  Next Article 

Journal of Virology, January 2006, p. 689-696, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.689-696.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Defining the Cellular Target(s) of Porcine Reproductive and Respiratory Syndrome Virus Blocking Monoclonal Antibody 7G10

Jeong-Ki Kim,1 Al-Majhdi Fahad,1 Kumar Shanmukhappa,2 and Sanjay Kapil1*

Louise C. Averill Research Laboratory, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506,1 Division of Gastroenterology, Hepatology and Nutrition, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 422292

Received 15 June 2005/ Accepted 21 October 2005

We produced a monoclonal antibody (MAb) (7G10) that has blocking activity against porcine reproductive and respiratory syndrome virus (PRRSV). In this study, we identified the components of the 7G10 MAb-bound complex as cytoskeletal filaments: vimentin, cytokeratin 8, cytokeratin 18, actin, and hair type II basic keratin. Vimentin bound to PRRSV nucleocapsid protein and anti-vimentin antibodies showed PRRSV-blocking activity. Vimentin was expressed on the surface of MARC-145, a PRRSV-susceptible cell line. Simian vimentin rendered BHK-21 and CRFK, nonsusceptible cell lines, susceptible to PRRSV infection. These results suggest that vimentin is part of the PRRSV receptor complex and that it plays an important role in PRRSV binding with the other cytoskeletal filaments that mediate transportation of the virus in the cytosol.

* Corresponding author. Mailing address: Louise C. Averill Research Laboratory, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506. Phone: (785) 532-4457. Fax: (785) 532-4829. E-mail: kapil{at}vet.ksu.edu.

Journal of Virology, January 2006, p. 689-696, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.689-696.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Miller, M. S., Hertel, L. (2009). Onset of Human Cytomegalovirus Replication in Fibroblasts Requires the Presence of an Intact Vimentin Cytoskeleton. J. Virol. 83: 7015-7028 [Abstract] [Full Text]  
  • Van Gorp, H., Van Breedam, W., Delputte, P. L., Nauwynck, H. J. (2008). Sialoadhesin and CD163 join forces during entry of the porcine reproductive and respiratory syndrome virus. J. Gen. Virol. 89: 2943-2953 [Abstract] [Full Text]  
  • Delputte, P. L., Van Breedam, W., Delrue, I., Oetke, C., Crocker, P. R., Nauwynck, H. J. (2007). Porcine Arterivirus Attachment to the Macrophage-Specific Receptor Sialoadhesin Is Dependent on the Sialic Acid-Binding Activity of the N-Terminal Immunoglobulin Domain of Sialoadhesin. J. Virol. 81: 9546-9550 [Abstract] [Full Text]  
  • Calvert, J. G., Slade, D. E., Shields, S. L., Jolie, R., Mannan, R. M., Ankenbauer, R. G., Welch, S.-K. W. (2007). CD163 Expression Confers Susceptibility to Porcine Reproductive and Respiratory Syndrome Viruses. J. Virol. 81: 7371-7379 [Abstract] [Full Text]  
  • Costers, S., Delputte, P. L., Nauwynck, H. J. (2006). Porcine reproductive and respiratory syndrome virus-infected alveolar macrophages contain no detectable levels of viral proteins in their plasma membrane and are protected against antibody-dependent, complement-mediated cell lysis.. J. Gen. Virol. 87: 2341-2351 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»