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Journal of Virology, January 2006, p. 654-662, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.654-662.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Transcription Profile of Aleutian Mink Disease Virus in CRFK Cells Is Generated by Alternative Processing of Pre-mRNAs Produced from a Single Promoter

Jianming Qiu,* Fang Cheng, Lisa R. Burger, and David Pintel

Department of Molecular Microbiology and Immunology, Life Sciences Center, University of Missouri—Columbia, School of Medicine, Columbia, Missouri 65211

Received 29 July 2005/ Accepted 21 October 2005

A reevaluation of the transcription profile of Aleutian mink disease parvovirus (AMDV)-infected CRFK cells at either 32°C or 37°C has determined that strain AMDV-G encodes six species of mRNAs produced by alternative splicing and alternative polyadenylation of a pre-mRNA generated by a single promoter at the left end of the genome. Three different splicing patterns are used, and each type is found polyadenylated at either the 3' end of the genome (the distal site) or at a site in the center of the genome (the proximal site). All spliced species accumulate similarly over the course of infection, with the R2 RNA predominant throughout. The R2 RNA, which contains and can express the NS2 coding region, encodes the viral capsid proteins VP1 and VP2.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Immunology, University of Missouri—Columbia, School of Medicine, 471b Life Sciences Center, 1201 E. Rollins Rd., Columbia, MO 65211-7310. Phone: (573) 882-3171. Fax: (573) 884-9395. E-mail: qiuj{at}missouri.edu.

Journal of Virology, January 2006, p. 654-662, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.654-662.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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