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Journal of Virology, October 2006, p. 9910-9915, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.01247-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of a 709-Amino-Acid Internal Nonessential Region within the Essential Conserved Tegument Protein (p)UL36 of Pseudorabies Virus

Sindy Böttcher,1 Barbara G. Klupp,1 Harald Granzow,2 Walter Fuchs,1 Kathrin Michael,1 and Thomas C. Mettenleiter1*

Institutes of Molecular Biology,1 Infectology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany2

Received 14 June 2006/ Accepted 11 July 2006

Tegument proteins homologous to the essential herpes simplex virus type 1 UL36 gene product (p)UL36 are conserved throughout the Herpesviridae and constitute the largest herpesvirus-encoded proteins. So far, only limited information is available on their functions, which include complex formation with the (p)UL37 homologs via an N-terminal domain and a deubiquitinating activity in the extreme N terminus. For further analysis we constructed deletion mutants lacking 437, 784, 926, 1,046, 1,217, or 1,557 amino acids (aa) from the C terminus. While none of them supported replication of a pseudorabies virus (PrV) UL36 deletion mutant, a mutant polypeptide with an internal deletion from aa 2087 to 2795, which comprises a proline/alanine-rich region, fully complemented the lethal replication defect. Thus, our data indicate that the extreme C terminus of (p)UL36 fulfills an essential role in PrV replication, while a large internal portion of the C-terminal half of the protein is dispensable for replication in cell culture.


* Corresponding author. Mailing address: Friedrich-Loeffler-Institut, Institute of Molecular Biology, Boddenblick 5A, 17493 Greifswald-Insel Riems, Germany. Phone: 49-38351-7250. Fax: 49-38351-7151. E-mail: thomas.mettenleiter{at}fli.bund.de.


Journal of Virology, October 2006, p. 9910-9915, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.01247-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

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