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Journal of Virology, October 2006, p. 9899-9904, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.00640-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Regulation of the Transcription and Replication Cycle of Human Cytomegalovirus Is Insensitive to Genetic Elimination of the Cognate NF-B Binding Sites in the Enhancer

Montse Gustems,¹ Eva Borst,^2,3 Chris A. Benedict,⁴ Carmen Pérez,¹ Martin Messerle,² Peter Ghazal,⁵ and Ana Angulo^1*

Institut d'Investigacions Biomèdiques August Pi i Sunyer, 08036 Barcelona, Spain,¹ Department of Virology, Hannover Medical School, 30625 Hannover, Germany,² Medical Faculty, University of Halle, 06120 Halle, Germany,³ Division of Molecular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121,⁴ Scottish Centre for Genomic Technology and Informatics, University of Edinburgh, Edinburgh EH16 4SB, United Kingdom⁵

Received 30 March 2006/ Accepted 10 July 2006

The role of NF-B in regulating human cytomegalovirus (HCMV) replication and gene transcription remains controversial. Multiple, functional NF-B response elements exist in the major immediate-early promoter (MIEP) enhancer of HCMV, suggesting a possible requirement for this transcription factor in lytic viral replication. Here we demonstrate by generating and analyzing HCMVs with alterations in the MIEP-enhancer that, although this region is essential for HCMV growth, none of the four NF-B response elements contained within the enhancer are required for MIE gene expression or HCMV replication in multiple cell types. These data reveal the robustness of the regulatory network controlling the MIEP enhancer.

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* Corresponding author. Mailing address: Institut d'Investigacions Biomèdiques August Pi i Sunyer, C/ Villarroel 170, Barcelona 08036, Spain. Phone: 34 647 450269. Fax: 34 93 4021907. E-mail: aangulo{at}ub.edu.

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Journal of Virology, October 2006, p. 9899-9904, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.00640-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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