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Journal of Virology, October 2006, p. 9889-9895, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.00664-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mobilization of Full-Length Semliki Forest Virus Replicon by Retrovirus Particles

Eric Piver,1 Christine Collin,2 Noémie Renault,1,{dagger} Thierry Bru,1,{ddagger} and Jean-Christophe Pagès1*

Université François Rabelais, INSERM ERI 19, EA 3856-IFR 136, Faculté de Médecine de Tours, 10 Bd Tonnellé, Tours, France,1 Service de Biochimie et Biologie Moléculaire, CHRU Trousseau, Tours, France2

Received 3 April 2006/ Accepted 8 July 2006

Conciliating biosafety with efficient gene transfer remains a constant concern in the development of retroviral vectors. Semliki Forest virus (SFV) replicons allow important retroviral vector production with interesting features. It is noteworthy that retroviruses have the ability to package {Psi}+ and, to some extent, {Psi}– cellular RNAs. Therefore, it was important to study the retroviral transfer of highly abundant SFV genomes expressing retroviral proteins. Here, we show that full-length SFV-vector replicons, with or without {Psi}, are efficiently packaged into retrovirus particles. Mechanistically, our data suggest that SFV packaging is the sum of its retroviral nucleocapsid-dependent recruitment together with a passive hijacking of membrane-anchored SFV replicon. A direct consequence of this phenomenon is the formation of particles harboring autonomous replicative abilities and contaminating vector preparations. Importantly, we confirm that retroviral SFV mobilization is not an exclusive feature of murine gamma retroviruses, since it is also observed using lentivectors.

* Corresponding author. Mailing address: Université François Rabelais, INSERM ERI 19, EA 3856-IFR 136, Faculté de Médecine de Tours, 10 Bd Tonnellé, Tours, France. Phone: 33 2 47 47 87 68. Fax: 33 2 47 36 61 26. E-mail: pages{at}med.univ-tours.fr.

{dagger} Present address: UMR CNRS 7151 Hôpital Saint-Louis, Paris, France.

{ddagger} Present address: The Wellcome Trust, Dundee, United Kingdom.

Journal of Virology, October 2006, p. 9889-9895, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.00664-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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