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Journal of Virology, October 2006, p. 9511-9518, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.01035-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mapping and Functional Role of the Self-Association Domain of Vesicular Stomatitis Virus Phosphoprotein

Mingzhou Chen, Tomoaki Ogino, and Amiya K. Banerjee*

Virology Section, Department of Molecular Genetics, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195

Received 19 May 2006/ Accepted 6 July 2006

The phosphoprotein (P protein) of vesicular stomatitis virus (VSV) is an essential subunit of the viral RNA-dependent RNA polymerase complex and plays a central role in viral transcription and replication. Using both the yeast two-hybrid system and coimmunoprecipitation assays, we confirmed the self-association of the P protein of Indiana serotype (Pind) and heterotypic interaction between Pind and the P protein of New Jersey serotype (Pnj). Furthermore, by using various truncation and deletion mutants of Pind, the self-association domain of the Pind protein was mapped to amino acids 161 to 210 within the hinge region. The self-association domain of Pind protein is not required for its binding to nucleocapsid and large proteins. We further demonstrated that the self-association domain of Pind protein is essential for VSV transcription in a minireplicon system and that a synthetic peptide spanning amino acids 191 to 210 in the self-association domain of Pind protein strongly inhibited the transcription of the VSV genome in vitro in a dose-dependent manner. These results indicated that the self-association domain of Pind protein plays a critical role in VSV transcription.

* Corresponding author. Mailing address: Virology Section, Department of Molecular Genetics, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195. Phone: (216) 444-0625. Fax: (216) 444-2998. E-mail: banerja{at}ccf.org.

Journal of Virology, October 2006, p. 9511-9518, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.01035-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Green, T. J., Luo, M. (2009). Structure of the vesicular stomatitis virus nucleocapsid in complex with the nucleocapsid-binding domain of the small polymerase cofactor, P. Proc. Natl. Acad. Sci. USA 106: 11713-11718 [Abstract] [Full Text]  
  • Chen, M., Ogino, T., Banerjee, A. K. (2007). Interaction of Vesicular Stomatitis Virus P and N Proteins: Identification of Two Overlapping Domains at the N Terminus of P That Are Involved in N0-P Complex Formation and Encapsidation of Viral Genome RNA. J. Virol. 81: 13478-13485 [Abstract] [Full Text]  


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