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Journal of Virology, September 2006, p. 9331-9335, Vol. 80, No. 18
0022-538X/06/$08.00+0     doi:10.1128/JVI.01160-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Crystal Structure of the Receptor-Binding Protein Head Domain from Lactococcus lactis Phage bIL170

Stefano Ricagno,1 Valérie Campanacci,1 Stéphanie Blangy,1 Silvia Spinelli,1 Denise Tremblay,2,3 Sylvain Moineau,2,3,4 Mariella Tegoni,1 and Christian Cambillau1*

Architecture et Fonction des Macromolécules Biologiques, UMR 6098 CNRS and Universités Aix-Marseille I & II, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France,1 Groupe de Recherche en Écologie Buccale (GREB), Faculté de Médecine Dentaire,2 Félix d'Hérelle Reference Center for Bacterial Viruses,3 Département de Biochimie et de Microbiologie, Faculté des Sciences et de Génie, Université Laval, Québec City, Québec, Canada G1K 7P44

Received 5 June 2006/ Accepted 21 June 2006

Lactococcus lactis, a gram-positive bacterium widely used by the dairy industry, is subject to lytic phage infections. In the first step of infection, phages recognize the host saccharidic receptor using their receptor binding protein (RBP). Here, we report the 2.30-Å-resolution crystal structure of the RBP head domain from phage bIL170. The structure of the head monomer is remarkably close to those of other lactococcal phages, p2 and TP901-1, despite any sequence identity with them. The knowledge of the three-dimensional structures of three RBPs gives a better insight into the module exchanges which have occurred among phages.

* Corresponding author. Mailing address: Architecture et Fonction des Macromolécules Biologiques, UMR 6098 CNRS and Universités Aix-Marseille I & II, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France. Phone: 33 491 825 590. Fax: 33 491 266 720. E-mail: cambillau{at}afmb.univ-mrs.fr.

Journal of Virology, September 2006, p. 9331-9335, Vol. 80, No. 18
0022-538X/06/$08.00+0     doi:10.1128/JVI.01160-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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