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Journal of Virology, September 2006, p. 9270-9278, Vol. 80, No. 18
0022-538X/06/$08.00+0     doi:10.1128/JVI.00536-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genome Characterization of Lipid-Containing Marine Bacteriophage PM2 by Transposon Insertion Mutagenesis

Mart Krupovic,1 Heikki Vilen,2 Jaana K. H. Bamford,1,{dagger} Hanna M. Kivelä,1 Juha-Matti Aalto,2 Harri Savilahti,2 and Dennis H. Bamford1*

Department of Biological and Environmental Sciences and Institute of Biotechnology, Biocenter 2, P.O. Box 56 (Viikinkaari 5), FIN-00014 University of Helsinki, Finland,1 Institute of Biotechnology, Biocenter 1, P.O. Box 56 (Viikinkaari 9), FIN-00014 University of Helsinki, Finland2

Received 15 March 2006/ Accepted 27 June 2006

Bacteriophage PM2 presently is the only member of the Corticoviridae family. The virion consists of a protein-rich lipid vesicle, which is surrounded by an icosahedral protein capsid. The lipid vesicle encloses a supercoiled circular double-stranded DNA genome of 10,079 bp. PM2 belongs to the marine phage community and is known to infect two gram-negative Pseudoalteromonas species. In this study, we present a characterization of the PM2 genome made using the in vitro transposon insertion mutagenesis approach. Analysis of 101 insertion mutants yielded information on the essential and dispensable regions of the PM2 genome and led to the identification of several new genes. A number of lysis-deficient mutants as well as mutants displaying delayed- and/or incomplete-lysis phenotypes were identified. This enabled us to identify novel lysis-associated genes with no resemblance to those previously described from other bacteriophage systems. Nonessential genome regions are discussed in the context of PM2 genome evolution.

* Corresponding author. Mailing address: Viikki Biocenter, P.O. Box 56 (Viikinkaari 5), FIN-00014 University of Helsinki, Finland. Phone: 358 9 191 59100. Fax: 358 9 191 59098. E-mail: dennis.bamford{at}helsinki.fi.

{dagger} Present address: Department of Biological and Environmental Science, P.O. Box 35, FIN-40014 University of Jyväskylä, Finland.

Journal of Virology, September 2006, p. 9270-9278, Vol. 80, No. 18
0022-538X/06/$08.00+0     doi:10.1128/JVI.00536-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Kivela, H. M., Madonna, S., Krupovic, M., Tutino, M. L., Bamford, J. K. H. (2008). Genetics for Pseudoalteromonas Provides Tools To Manipulate Marine Bacterial Virus PM2. J. Bacteriol. 190: 1298-1307 [Abstract] [Full Text]  


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