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Journal of Virology, September 2006, p. 8830-8833, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00880-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Functional Entry of Baculovirus into Insect and Mammalian Cells Is Dependent on Clathrin-Mediated Endocytosis

Gang Long,1,2 Xiaoyu Pan,1 Richard Kormelink,2 and Just M. Vlak2*

State Key Laboratory of Virology, Key Laboratory of Molecular Virology and Joint Lab of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China,1 Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands2

Received 1 May 2006/ Accepted 22 May 2006

Entry of the budded virus form of baculoviruses into insect and mammalian cells is generally thought to occur through a low-pH-dependent endocytosis pathway, possibly through clathrin-coated pits. This insight is primarily based on (immuno)electron microscopy studies but requires biochemical support to exclude the use of other pathways. Here, we demonstrate using various inhibitors that functional entry of baculoviruses into insect and mammalian cells is primarily dependent on clathrin-mediated endocytosis. Our results further suggest that caveolae are somehow involved in baculovirus entry in mammalian cells. A caveolar endocytosis inhibitor, genistein, enhances baculovirus transduction in these cells considerably.

* Corresponding author. Mailing address: Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands. Phone: 31-317-483090. Fax: 31-317-484820. E-mail: just.vlak{at}wur.nl.

Journal of Virology, September 2006, p. 8830-8833, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00880-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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