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Journal of Virology, September 2006, p. 8593-8602, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00537-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Spread of Rice Dwarf Virus among Cells of Its Insect Vector Exploits Virus-Induced Tubular Structures

Taiyun Wei,1 Akira Kikuchi,1,{dagger} Yusuke Moriyasu,1,2 Nobuhiro Suzuki,3 Takumi Shimizu,1 Kyoji Hagiwara,1 Hongyan Chen,1,5 Mami Takahashi,4,{ddagger} Tamaki Ichiki-Uehara,1 and Toshihiro Omura1*

Laboratory of Virology, National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan,1 Faculty of Agriculture, Ibaraki University, Ami, Ibaraki 300-0332, Japan,2 Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan,3 National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan,4 Biotechnology Center, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, People's Republic of China5

Received 15 March 2006/ Accepted 4 June 2006

Various cytopathological structures, known as inclusion bodies, are formed upon infection of cultured leafhopper cells by Rice dwarf virus, a member of the family Reoviridae. These structures include tubules of approximately 85 nm in diameter which are composed of the nonstructural viral protein Pns10 and contain viral particles. Such tubular structures were produced in heterologous non-host insect cells that expressed Pns10 of the virus. These tubules, when associated with actin-based filopodia, were able to protrude from the surface of cells and to penetrate neighboring cells. A binding assay in vitro revealed the specific binding of Pns10 to actin. Infection of clusters of cells was readily apparent 5 days after inoculation at a low multiplicity of infection with the virus, even in the presence of neutralizing antibodies. However, treatment of host cells with drugs that inhibited the elongation of actin filaments abolished the extension of Pns10 tubules from the surface of cells, with a significant simultaneous decrease in the extent of infection of neighboring cells. These results together revealed a previously undescribed aspect of the intercellular spread of Rice dwarf virus, wherein the virus exploits tubules composed of a nonstructural viral protein and actin-based filopodia to move into neighboring cells.

* Corresponding author. Mailing address: National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan. Phone: 81 29 838 8932. Fax: 81 29 838 8929. E-mail: toomura{at}affrc.go.jp.

{dagger} Present address: Gene Experiment Center, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.

{ddagger} Present address: National Agricultural Research Center, 1-2-1 Inada, Joetsu, Niigata 943-0193, Japan.

Journal of Virology, September 2006, p. 8593-8602, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00537-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wei, T., Hibino, H., Omura, T. (2008). Rice dwarf virus is engulfed into and released via vesicular compartments in cultured insect vector cells. J. Gen. Virol. 89: 2915-2920 [Abstract] [Full Text]  
  • Zhou, F., Pu, Y., Wei, T., Liu, H., Deng, W., Wei, C., Ding, B., Omura, T., Li, Y. (2007). The P2 capsid protein of the nonenveloped rice dwarf phytoreovirus induces membrane fusion in insect host cells. Proc. Natl. Acad. Sci. USA 104: 19547-19552 [Abstract] [Full Text]  
  • Katayama, S., Wei, T., Omura, T., Takagi, J., Iwasaki, K. (2007). Three-Dimensional Architecture of Virus-Packed Tubule. J Electron Microsc (Tokyo) 56: 77-81 [Abstract] [Full Text]  


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