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Journal of Virology, August 2006, p. 8274-8278, Vol. 80, No. 16
0022-538X/06/$08.00+0     doi:10.1128/JVI.00476-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

NSm and 78-Kilodalton Proteins of Rift Valley Fever Virus Are Nonessential for Viral Replication in Cell Culture

Sungyong Won,1 Tetsuro Ikegami,1 C. J. Peters,1,2 and Shinji Makino1*

Department of Microbiology and Immunology,1 Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-10192

Received 7 March 2006/ Accepted 30 May 2006

Rift Valley fever viruses carrying mutations of the M gene preglycoprotein region, one lacking NSm protein expression, one lacking 78-kDa protein expression, and one lacking expression of both proteins, were compared in cell culture. All of the mutants and their parent virus produced plaques with similar sizes and morphologies in Vero E6 cells and had similar growth kinetics in Vero, C6/36, and MRC5 cells, demonstrating that the NSm and 78-kDa proteins were not needed for the virus to replicate efficiently in cell culture. A competition-propagation assay revealed that the parental virus was slightly more fit than the mutant virus lacking expression of both proteins.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019. Phone: (409) 772-2323. Fax: (409) 772-5065. E-mail: shmakino{at}utmb.edu.

Journal of Virology, August 2006, p. 8274-8278, Vol. 80, No. 16
0022-538X/06/$08.00+0     doi:10.1128/JVI.00476-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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