Journal of Virology, August 2006, p. 8259-8262, Vol. 80, No. 16
0022-538X/06/$08.00+0 doi:10.1128/JVI.00776-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
The L2 Minor Capsid Protein of Low-Risk Human Papillomavirus Type 11 Interacts with Host Nuclear Import Receptors and Viral DNA
J. Bordeaux,
S. Forte,
E. Harding,
M. S. Darshan,
K. Klucevsek, and
J. Moroianu*
Biology Department, Boston College, Chestnut Hill, Massachusetts 02467
Received 17 April 2006/
Accepted 30 May 2006
Analysis of the interactions of low-risk human papillomavirus type 11 (HPV11) L2 with karyopherin ß (Kap ß) nuclear import receptors revealed that L2 interacted with Kap ß1, Kap ß2, and Kap ß3 and formed a complex with the Kap
2ß1 heterodimer. HPV11 L2 contains two nuclear localization signals (NLSs)in the N terminus and the C terminusthat could mediate its nuclear import via a classical pathway. Each NLS was functional in vivo, and deletion of both of them abolished L2 nuclear localization. Both NLSs interacted with the viral DNA. Thus, HPV11 L2 can interact with several karyopherins and the viral DNA and may enter the nucleus via multiple pathways.
* Corresponding author. Mailing address: Boston College, Biology Department, Higgins Hall, Room 578, 140 Commonwealth Avenue, Chestnut Hill, MA 02467. Phone: (617) 552-1713. Fax: (617) 552-2011. E-mail: moroianu{at}bc.edu.
Journal of Virology, August 2006, p. 8259-8262, Vol. 80, No. 16
0022-538X/06/$08.00+0 doi:10.1128/JVI.00776-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
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Copyright © 2006 by the American Society for Microbiology. All rights reserved.