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Journal of Virology, August 2006, p. 7781-7785, Vol. 80, No. 15
0022-538X/06/$08.00+0     doi:10.1128/JVI.00481-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A Common Mechanism for Cytoplasmic Dynein-Dependent Microtubule Binding Shared among Adeno-Associated Virus and Adenovirus Serotypes

Samir Kelkar,1,2 Bishnu P. De,1 Guangping Gao,3 James M. Wilson,3 Ronald G. Crystal,1 and Philip L. Leopold1*

Department of Genetic Medicine, Weill Medical College of Cornell University, New York, New York,1 Graduate Program in Physiology and Biophysics and Systems Biology, Weill Medical College of Cornell University, New York, New York,2 Division of Medical Genetics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania3

Received 7 March 2006/ Accepted 15 April 2006

During infection, adenovirus-associated virus (AAV) undergoes microtubule-dependent retrograde transport as part of a program of vectorial transport of viral genome to the nucleus. A microtubule binding assay was used to evaluate the hypothesis that cytoplasmic dynein mediates AAV interaction with microtubules. Binding of AAV serotype 2 (AAV2) was enhanced in a nucleotide-dependent manner by the presence of total cellular microtubule-associated proteins (MAPs) but not cytoplasmic dynein-depleted MAPs. Excess AAV2 capsid protein prevented microtubule binding by AAV serotypes 2, 5, and rh.10, as well as adenovirus serotype 5, indicating that similar binding sites are used by these viruses.

* Corresponding author. Mailing address: Weill Medical College of Cornell University, Department of Genetic Medicine, 515 E. 71st Street, S-1000, New York, NY 10021. Phone: (212) 746-2258. Fax: (212) 746-8383. E-mail: geneticmedicine{at}med.cornell.edu.

Journal of Virology, August 2006, p. 7781-7785, Vol. 80, No. 15
0022-538X/06/$08.00+0     doi:10.1128/JVI.00481-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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