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Journal of Virology, August 2006, p. 7765-7768, Vol. 80, No. 15
0022-538X/06/$08.00+0     doi:10.1128/JVI.00542-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Integration of Human Immunodeficiency Virus Type 1 in Untreated Infection Occurs Preferentially within Genes

Hongbing Liu,1 Eugene C. Dow,1 Reetakshi Arora,1 Jason T. Kimata,1 Lara M. Bull,2 Roberto C. Arduino,2 and Andrew P. Rice1*

Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas,1 Section of Infectious Diseases, Department of Medicine, University of Texas Health Science Center at Houston School of Medicine, Houston, Texas2

Received 15 March 2006/ Accepted 11 May 2006

Previous analyses of human immunodeficiency virus type 1 (HIV-1) integration sites generated in infections in vitro or in patients in whom viral replication was repressed by antiviral therapy have demonstrated a preference for integration within protein-coding genes. We analyzed integration sites in peripheral blood mononuclear cells (PBMCs), spleen, lymph node, and cerebral cortex from patients with untreated HIV-1 infections. The great majority of integration sites in each tissue were within genes. Statistical analyses of the frequencies of integration in genes in PBMCs and lymph tissue demonstrated a strong preference for integration within genes. Although the sample size for brain tissue was too small to demonstrate a clear statistical preference for integration in genes, four of the five integration sites identified in brain were within genes. Taken together, our data indicate that HIV-1 preferentially integrates within genes during untreated infection.

* Corresponding author. Mailing address: Baylor College of Medicine, Department of Molecular Virology and Microbiology, One Baylor Plaza, Houston, TX 77030. Phone: (713) 798-5774. Fax: (713) 798-3490. E-mail: arice{at}bcm.tmc.edu.

Journal of Virology, August 2006, p. 7765-7768, Vol. 80, No. 15
0022-538X/06/$08.00+0     doi:10.1128/JVI.00542-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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