This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Allen, M. J.
Right arrow Articles by Wilson, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Allen, M. J.
Right arrow Articles by Wilson, W. H.

 Previous Article  |  Next Article 

Journal of Virology, August 2006, p. 7699-7705, Vol. 80, No. 15
0022-538X/06/$08.00+0     doi:10.1128/JVI.00491-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Locus-Specific Gene Expression Pattern Suggests a Unique Propagation Strategy for a Giant Algal Virus{dagger}

Michael J. Allen,1 Thorsten Forster,2 Declan C. Schroeder,3 Matthew Hall,3 Douglas Roy,2 Peter Ghazal,2 and William H. Wilson*1

Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, United Kingdom,1 Scottish Centre for Genomic Technology and Informatics, University of Edinburgh, Chancellor's Building, College of Medicine, 49 Little France Crescent, Edinburgh, EH16 4SB, United Kingdom,2 Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB, United Kingdom3

Received 9 March 2006/ Accepted 10 May 2006

Emiliania huxleyi virus strain 86 is the largest algal virus sequenced to date and is unique among the Phycodnaviridae since its genome is predicted to contain six RNA polymerase subunit genes. We have used a virus microarray to profile the temporal transcription strategy of this unusual virus during infection. There are two distinct transcription phases to the infection process. The primary phase is dominated by a group of coding sequences (CDSs) expressed by 1 h postinfection that are localized to a subregion of the genome. The CDS of the primary group have no database homologues, and each is associated with a unique promoter element. The remainder of the CDSs are expressed in a secondary phase between 2 and 4 hours postinfection. Compartmentalized transcription of the two distinctive phases is discussed. We hypothesize that immediately after infection the nucleic acid of the virus targets the host nucleus, where primary-phase genes are transcribed by host RNA polymerase which recognizes the viral promoter. Secondary-phase transcription may then be conducted in the cytoplasm.

* Corresponding author. Mailing address: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, United Kingdom. Phone: 44 1752 633100. Fax: 44 01752 633101. E-mail: whw{at}pml.ac.uk.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, August 2006, p. 7699-7705, Vol. 80, No. 15
0022-538X/06/$08.00+0     doi:10.1128/JVI.00491-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vardi, A., Van Mooy, B. A. S., Fredricks, H. F., Popendorf, K. J., Ossolinski, J. E., Haramaty, L., Bidle, K. D. (2009). Viral Glycosphingolipids Induce Lytic Infection and Cell Death in Marine Phytoplankton. Science 326: 861-865 [Abstract] [Full Text]  
  • Monier, A., Pagarete, A., de Vargas, C., Allen, M. J., Read, B., Claverie, J.-M., Ogata, H. (2009). Horizontal gene transfer of an entire metabolic pathway between a eukaryotic alga and its DNA virus. Genome Res 19: 1441-1449 [Abstract] [Full Text]  
  • Ikushiro, H., Islam, M. M., Tojo, H., Hayashi, H. (2007). Molecular Characterization of Membrane-Associated Soluble Serine Palmitoyltransferases from Sphingobacterium multivorum and Bdellovibrio stolpii. J. Bacteriol. 189: 5749-5761 [Abstract] [Full Text]  
  • Bidle, K. D., Haramaty, L., Barcelos e Ramos, J., Falkowski, P. (2007). Viral activation and recruitment of metacaspases in the unicellular coccolithophore, Emiliania huxleyi. Proc. Natl. Acad. Sci. USA 104: 6049-6054 [Abstract] [Full Text]  
  • Han, G., Gable, K., Yan, L., Allen, M. J., Wilson, W. H., Moitra, P., Harmon, J. M., Dunn, T. M. (2006). Expression of a Novel Marine Viral Single-chain Serine Palmitoyltransferase and Construction of Yeast and Mammalian Single-chain Chimera. J. Biol. Chem. 281: 39935-39942 [Abstract] [Full Text]  
  • Allen, M. J., Wilson, W. H. (2006). The coccolithovirus microarray: an array of uses. Brief Funct Genomic Proteomic 5: 273-279 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»