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Journal of Virology, July 2006, p. 7260-7264, Vol. 80, No. 14
0022-538X/06/$08.00+0     doi:10.1128/JVI.00051-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Infection of Naïve Target Cells with Virus-Like Particles: Implications for the Function of Ebola Virus VP24

Thomas Hoenen,^1,2 Allison Groseth,^2,3 Larissa Kolesnikova,¹ Steven Theriault,^2,3 Hideki Ebihara,^4,5 Bettina Hartlieb,¹ Sandra Bamberg,¹ Heinz Feldmann,^2,3 Ute Ströher,^2,3 and Stephan Becker^1*

Institut für Virologie, Philipps Universität Marburg, Marburg, Germany,¹ National Laboratory for Zoonotic Diseases and Special Pathogens, National Microbiology Laboratory, Public Health Agency of Canada, Manitoba, Canada,² Department of Medical Microbiology, University of Manitoba, Manitoba, Canada,³ Institute of Medical Science, University of Tokyo, Tokyo, Japan,⁴ CREST, Japan Science and Technology Corporation, Saitama, Japan⁵

Received 9 January 2006/ Accepted 8 April 2006

Infectious virus-like particle (iVLP) systems have recently been established for several negative-strand RNA viruses, including the highly pathogenic Zaire ebolavirus (ZEBOV), and allow study of the viral life cycle under biosafety level 2 conditions. However, current systems depend on the expression of viral helper nucleocapsid proteins in target cells, thus making it impossible to determine whether ribonucleoprotein complexes transferred by iVLPs are able to facilitate initial transcription, an indispensable step in natural infection. Here we describe a ZEBOV iVLP system which overcomes this limitation and show that VP24 is essential for the formation of a functional ribonucleoprotein complex.

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* Corresponding author. Mailing address: Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany. Phone: 49 30 4547-2373. Fax: 49 30 4547-2328. E-mail: BeckerSt{at}rki.de.

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Journal of Virology, July 2006, p. 7260-7264, Vol. 80, No. 14
0022-538X/06/$08.00+0     doi:10.1128/JVI.00051-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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