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Journal of Virology, July 2006, p. 6706-6711, Vol. 80, No. 13
0022-538X/06/$08.00+0     doi:10.1128/JVI.00273-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

High Rate of Genetic Recombination in Murine Leukemia Virus: Implications for Influencing Proviral Ploidy

Jianling Zhuang,1,{dagger} Sayandip Mukherjee,1,2,{dagger} Yacov Ron,1 and Joseph P. Dougherty1*

Department of Molecular Genetics, Microbiology and Immunology, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854,1 Graduate Program in Molecular Biosciences, Rutgers University, New Brunswick, New Jersey 089012

Received 7 February 2006/ Accepted 12 April 2006

A significant difference in the recombination rates between human immunodeficiency virus type 1 (HIV-1) and the gammaretroviruses was previously reported, with the former being 10 to 100 times more recombinogenic. It is possible that preferential copackaging of homodimers in the case of gammaretroviruses, like murine leukemia virus (MLV), led to the underestimation of their rates of recombination. To reexamine the recombination rates for MLV, experiments were performed to control for nonrandom copackaging of viral RNA, and it was found that MLV and HIV-1 exhibit similar crossover rates. The implications for control of proviral ploidy and preferential recombination during minus-strand DNA synthesis are discussed.

* Corresponding author. Mailing address: UMDNJ, Robert W. Johnson Medical School, Dept. of Molecular Genetics, Microbiology and Immunology, 675 Hoes Lane, Piscataway, NJ 08854-5635. Phone: (732) 235-4588. Fax: (732) 235-5223. E-mail: doughejp{at}umdnj.edu.

{dagger} These authors contributed equally to the work.

Journal of Virology, July 2006, p. 6706-6711, Vol. 80, No. 13
0022-538X/06/$08.00+0     doi:10.1128/JVI.00273-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Duggal, N. K., Goo, L., King, S. R., Telesnitsky, A. (2007). Effects of Identity Minimization on Moloney Murine Leukemia Virus Template Recognition and Frequent Tertiary Template-Directed Insertions during Nonhomologous Recombination. J. Virol. 81: 12156-12168 [Abstract] [Full Text]  
  • Mukherjee, S., Lee, H.-L. R., Ron, Y., Dougherty, J. P. (2006). Proviral Progeny of Heterodimeric Virions Reveal a High Crossover Rate for Human Immunodeficiency Virus Type 2. J. Virol. 80: 12402-12407 [Abstract] [Full Text]  
  • Chen, J., Powell, D., Hu, W.-S. (2006). High Frequency of Genetic Recombination Is a Common Feature of Primate Lentivirus Replication. J. Virol. 80: 9651-9658 [Abstract] [Full Text]  


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