This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, G.
Right arrow Articles by Chen, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, G.
Right arrow Articles by Chen, J.

 Previous Article  |  Next Article 

Journal of Virology, July 2006, p. 6597-6602, Vol. 80, No. 13
0022-538X/06/$08.00+0     doi:10.1128/JVI.02078-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Recovery of Infectious Rabbit Hemorrhagic Disease Virus from Rabbits after Direct Inoculation with In Vitro-Transcribed RNA

Guangqing Liu,1 Yuying Zhang,1,2 Zheng Ni,1 Tao Yun,1 Zutian Sheng,1 Huali Liang,1 Jionggang Hua,1 Shuangmao Li,1 Qingyun Du,1 and Jianping Chen1*

Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,1 Faculty of Veterinary Medicine, Gansu Agriculture University, Lanzhou 730070, People's Republic of China2

Received 14 October 2005/ Accepted 22 March 2006

We report the first full-length infectious clone of strain JX/CHA/97 of rabbit hemorrhagic disease virus (RHDV). The transcripts from the full-length cDNA clones were infectious when they were directly injected into rabbits. The sequence of the virus recovered from the rabbits was identical to that of the injected RNA transcripts. The cDNA clone was engineered to contain one silent nucleotide change to create an EcoRV site (A to T at nucleotide 2908). The genetic marker was retained in the recovered progeny virus. The transfection of RNA transcripts into RK-13 cells resulted in the synthesis of viral antigens, indicating that the cDNA clones were replication competent. This stable infectious molecular clone should be an important tool for developing a better understanding of the molecular biology and pathogenesis of RHDV.

* Corresponding author. Mailing address: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, People's Republic of China. Phone: 86 571 86404241. Fax: 86 571 86404256. E-mail: jpchen2001{at}hzcnc.com.

Journal of Virology, July 2006, p. 6597-6602, Vol. 80, No. 13
0022-538X/06/$08.00+0     doi:10.1128/JVI.02078-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chen, L., Liu, G., Ni, Z., Yu, B., Yun, T., Song, Y., Hua, J., Li, S., Chen, J. (2009). Minor structural protein VP2 in rabbit hemorrhagic disease virus downregulates the expression of the viral capsid protein VP60. J. Gen. Virol. 90: 2952-2955 [Abstract] [Full Text]  
  • Liu, G., Ni, Z., Yun, T., Yu, B., Chen, L., Zhao, W., Hua, J., Chen, J. (2008). A DNA-launched reverse genetics system for rabbit hemorrhagic disease virus reveals that the VP2 protein is not essential for virus infectivity. J. Gen. Virol. 89: 3080-3085 [Abstract] [Full Text]  
  • Wei, C., Farkas, T., Sestak, K., Jiang, X. (2008). Recovery of Infectious Virus by Transfection of In Vitro-Generated RNA from Tulane Calicivirus cDNA. J. Virol. 82: 11429-11436 [Abstract] [Full Text]  
  • Chaudhry, Y., Skinner, M. A., Goodfellow, I. G. (2007). Recovery of genetically defined murine norovirus in tissue culture by using a fowlpox virus expressing T7 RNA polymerase. J. Gen. Virol. 88: 2091-2100 [Abstract] [Full Text]  
  • Ward, V. K., McCormick, C. J., Clarke, I. N., Salim, O., Wobus, C. E., Thackray, L. B., Virgin, H. W. IV, Lambden, P. R. (2007). Recovery of infectious murine norovirus using pol II-driven expression of full-length cDNA. Proc. Natl. Acad. Sci. USA 104: 11050-11055 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»