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Journal of Virology, July 2006, p. 6318-6323, Vol. 80, No. 13
0022-538X/06/$08.00+0     doi:10.1128/JVI.00427-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Poxvirus CD8+ T-Cell Determinants and Cross-Reactivity in BALB/c Mice

David C. Tscharke,1,2* Wai-Ping Woo,2,{dagger} Isaac G. Sakala,3 John Sidney,4 Alessandro Sette,4 Denis J. Moss,2 Jack R. Bennink,1 Gunasegaran Karupiah,3 and Jonathan W. Yewdell1*

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0440,1 Division of Immunology and Infectious Diseases, Queensland Institute of Medical Research, Herston QLD 4006, Australia,2 Division of Immunology and Genetics, John Curtin School of Medical Research, Australian National University, Canberra ACT 2601, Australia,3 Division of Translational Immunology and Biodefense, La Jolla Institute for Allergy and Immunology, San Diego, California 921214

Received 28 February 2006/ Accepted 18 April 2006

Mouse models of orthopoxvirus disease provide great promise for probing basic questions regarding host responses to this group of pathogens, which includes the causative agents of monkeypox and smallpox. However, some essential tools for their study that are taken for granted with other mouse models are not available for these viruses. Here we map and characterize the initial CD8+ T-cell determinants for poxviruses in H-2d-haplotype mice. CD8+ T cells recognizing these three determinants make up around 40% of the total responses to vaccinia virus during and after resolution of infection. We then use these determinants to test if predicted conservation across orthopoxvirus species matches experimental observation and find an unexpectedly cross-reactive variant peptide encoded by ectromelia (mousepox) virus.

* Corresponding author. Present address for David Tscharke: School of Biochemistry and Molecular Biology, Australian National University, Canberra ACT 0200, Australia. E-mail: david.tscharke{at}anu.edu.au. Mailing address for Jonathan Yewdell: Building 4, Room 211, 4 Center Drive, NIH, Bethesda, MD 20892-0440. Phone: (301) 402-4602. Fax: (301) 402-7362. E-mail: jyewdell{at}niaid.nih.gov.

{dagger} Present address: Coridon, Level 2 Research Extension Building 1, Princess Alexandra Hospital, Ipswich Rd., Buranda QLD 4102, Australia.

Journal of Virology, July 2006, p. 6318-6323, Vol. 80, No. 13
0022-538X/06/$08.00+0     doi:10.1128/JVI.00427-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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