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Journal of Virology, July 2006, p. 6198-6206, Vol. 80, No. 13
0022-538X/06/$08.00+0     doi:10.1128/JVI.00283-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Functional Replacement of the RING, B-Box 2, and Coiled-Coil Domains of Tripartite Motif 5{alpha} (TRIM5{alpha}) by Heterologous TRIM Domains

Xing Li,1 Yuan Li,1 Matthew Stremlau,1 Wen Yuan,1 Byeongwoon Song,1 Michel Perron,1 and Joseph Sodroski1,2*

Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Division of AIDS, Harvard Medical School,1 Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 021152

Received 8 February 2006/ Accepted 10 April 2006

Tripartite motif 5{alpha} (TRIM5{alpha}) restricts some retroviruses, including human immunodeficiency virus type 1 (HIV-1), from infecting the cells of particular species. TRIM5{alpha} is a member of the TRIM family of proteins, which contain RING, B-box, coiled-coil (CC), and, in some cases, B30.2(SPRY) domains. Here we investigated the abilities of domains from TRIM proteins (TRIM6, TRIM34, and TRIM21) that do not restrict HIV-1 infection to substitute for the domains of rhesus monkey TRIM5{alpha} (TRIM5{alpha}rh). The RING, B-box 2, and CC domains of the paralogous TRIM6 and TRIM34 proteins functionally replaced the corresponding TRIM5{alpha}rh domains, allowing HIV-1 restriction. By contrast, similar chimeras containing the components of TRIM21, a slightly more distant relative of TRIM5, did not restrict HIV-1 infection. The TRIM21 B-box 2 domain and its flanking linker regions contributed to the functional defectiveness of these chimeras. All of the chimeric proteins formed trimers. All of the chimeras that restricted HIV-1 infection bound the assembled HIV-1 capsid complexes. These results indicate that heterologous RING, B-box 2, and CC domains from related TRIM proteins can functionally substitute for TRIM5{alpha}rh domains.

* Corresponding author. Mailing address: Dana-Farber Cancer Institute, 44 Binney Street—JFB 824, Boston, MA 02115. Phone: (617) 632-3371. Fax: (671) 632-4338. E-mail: joseph_sodroski{at}dfci.harvard.edu.

Journal of Virology, July 2006, p. 6198-6206, Vol. 80, No. 13
0022-538X/06/$08.00+0     doi:10.1128/JVI.00283-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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