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Journal of Virology, June 2006, p. 6188-6191, Vol. 80, No. 12
0022-538X/06/$08.00+0     doi:10.1128/JVI.02676-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Human Cytomegalovirus (HCMV) UL82 Gene Product (pp71) Relieves hDaxx-Mediated Repression of HCMV Replication

Stacy R. Cantrell{dagger} and Wade A. Bresnahan*

Department of Microbiology, University of Minnesota, 420 Delaware St. SE, 1060 Mayo Building, MMC196, Minneapolis, Minnesota 55455

Received 21 December 2005/ Accepted 22 March 2006

This study examines the role of the cellular protein hDaxx in controlling human cytomegalovirus (HCMV) immediate-early (IE) gene expression and viral replication. Using permissive cell lines that either overexpress hDaxx or are depleted of hDaxx expression by the use of short hairpin RNA, we demonstrate that hDaxx functions as a repressor of HCMV IE gene expression and replication. In addition, we demonstrate that the impaired growth phenotype associated with the UL82 (pp71) deletion mutant is abolished when hDaxx knockdown cells are infected, suggesting that pp71 functions to relieve hDaxx-mediated repression during HCMV infection.

* Corresponding author. Mailing address: Department of Microbiology, University of Minnesota, 420 Delaware St. SE, 1060 Mayo Building, MMC196, Minneapolis, MN 55455. Phone: (612) 626-5876. Fax: (612) 626-0623. E-mail: bresn013{at}umn.edu.

{dagger} Present address: UT Southwestern Medical Center, Department of Microbiology, 6000 Harry Hines Blvd., Dallas, TX 75390.

Journal of Virology, June 2006, p. 6188-6191, Vol. 80, No. 12
0022-538X/06/$08.00+0     doi:10.1128/JVI.02676-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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