Previous Article | Next Article 
Journal of Virology, June 2006, p. 6188-6191, Vol. 80, No. 12
0022-538X/06/$08.00+0 doi:10.1128/JVI.02676-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Human Cytomegalovirus (HCMV) UL82 Gene Product (pp71) Relieves hDaxx-Mediated Repression of HCMV Replication
Stacy R. Cantrell
and
Wade A. Bresnahan*
Department of Microbiology, University of Minnesota, 420 Delaware St. SE, 1060 Mayo Building, MMC196, Minneapolis, Minnesota 55455
Received 21 December 2005/
Accepted 22 March 2006
This study examines the role of the cellular protein hDaxx in controlling human cytomegalovirus (HCMV) immediate-early (IE) gene expression and viral replication. Using permissive cell lines that either overexpress hDaxx or are depleted of hDaxx expression by the use of short hairpin RNA, we demonstrate that hDaxx functions as a repressor of HCMV IE gene expression and replication. In addition, we demonstrate that the impaired growth phenotype associated with the UL82 (pp71) deletion mutant is abolished when hDaxx knockdown cells are infected, suggesting that pp71 functions to relieve hDaxx-mediated repression during HCMV infection.
* Corresponding author. Mailing address: Department of Microbiology, University of Minnesota, 420 Delaware St. SE, 1060 Mayo Building, MMC196, Minneapolis, MN 55455. Phone: (612) 626-5876. Fax: (612) 626-0623. E-mail: bresn013{at}umn.edu.
Present address: UT Southwestern Medical Center, Department of Microbiology, 6000 Harry Hines Blvd., Dallas, TX 75390.
Journal of Virology, June 2006, p. 6188-6191, Vol. 80, No. 12
0022-538X/06/$08.00+0 doi:10.1128/JVI.02676-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Ullman, A. J., Hearing, P.
(2008). Cellular Proteins PML and Daxx Mediate an Innate Antiviral Defense Antagonized by the Adenovirus E4 ORF3 Protein. J. Virol.
82: 7325-7335
[Abstract]
[Full Text]
-
Kalejta, R. F.
(2008). Tegument Proteins of Human Cytomegalovirus. Microbiol. Mol. Biol. Rev.
72: 249-265
[Abstract]
[Full Text]
-
Everett, R. D., Parada, C., Gripon, P., Sirma, H., Orr, A.
(2008). Replication of ICP0-Null Mutant Herpes Simplex Virus Type 1 Is Restricted by both PML and Sp100. J. Virol.
82: 2661-2672
[Abstract]
[Full Text]
-
Poleshko, A., Palagin, I., Zhang, R., Boimel, P., Castagna, C., Adams, P. D., Skalka, A. M., Katz, R. A.
(2008). Identification of Cellular Proteins That Maintain Retroviral Epigenetic Silencing: Evidence for an Antiviral Response. J. Virol.
82: 2313-2323
[Abstract]
[Full Text]
-
Randall, R. E., Goodbourn, S.
(2008). Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures. J. Gen. Virol.
89: 1-47
[Abstract]
[Full Text]
-
Tavalai, N., Papior, P., Rechter, S., Stamminger, T.
(2008). Nuclear Domain 10 Components Promyelocytic Leukemia Protein and hDaxx Independently Contribute to an Intrinsic Antiviral Defense against Human Cytomegalovirus Infection. J. Virol.
82: 126-137
[Abstract]
[Full Text]
-
Groves, I. J., Sinclair, J. H.
(2007). Knockdown of hDaxx in normally non-permissive undifferentiated cells does not permit human cytomegalovirus immediate-early gene expression. J. Gen. Virol.
88: 2935-2940
[Abstract]
[Full Text]
-
Everett, R. D., Murray, J., Orr, A., Preston, C. M.
(2007). Herpes Simplex Virus Type 1 Genomes Are Associated with ND10 Nuclear Substructures in Quiescently Infected Human Fibroblasts. J. Virol.
81: 10991-11004
[Abstract]
[Full Text]
-
Sourvinos, G., Tavalai, N., Berndt, A., Spandidos, D. A., Stamminger, T.
(2007). Recruitment of Human Cytomegalovirus Immediate-Early 2 Protein onto Parental Viral Genomes in Association with ND10 in Live-Infected Cells. J. Virol.
81: 10123-10136
[Abstract]
[Full Text]
-
Saffert, R. T., Kalejta, R. F.
(2007). Human Cytomegalovirus Gene Expression Is Silenced by Daxx-Mediated Intrinsic Immune Defense in Model Latent Infections Established In Vitro. J. Virol.
81: 9109-9120
[Abstract]
[Full Text]
-
Katz, R. A., Jack-Scott, E., Narezkina, A., Palagin, I., Boimel, P., Kulkosky, J., Nicolas, E., Greger, J. G., Skalka, A. M.
(2007). High-Frequency Epigenetic Repression and Silencing of Retroviruses Can Be Antagonized by Histone Deacetylase Inhibitors and Transcriptional Activators, but Uniform Reactivation in Cell Clones Is Restricted by Additional Mechanisms. J. Virol.
81: 2592-2604
[Abstract]
[Full Text]
Copyright © 2006 by the American Society for Microbiology. All rights reserved.