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Journal of Virology, June 2006, p. 5733-5739, Vol. 80, No. 12
0022-538X/06/$08.00+0     doi:10.1128/JVI.00125-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Putative Terminase Subunit of Herpes Simplex Virus 1 Encoded by UL28 Is Necessary and Sufficient To Mediate Interaction between pUL15 and pUL33

Kui Yang and Joel D. Baines*

Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14850

Received 18 January 2006/ Accepted 5 April 2006

Viral terminases play essential roles as components of molecular motors that package viral DNA into capsids. Previous results indicated that the putative terminase subunits of herpes simplex virus 1 (HSV-1) encoded by UL15 and UL28 (designated pUL15 and pUL28, respectively) coimmunoprecipitate with the UL33 protein from lysates of infected cells. All three proteins are among six required for HSV-1 DNA packaging but dispensable for assembly of immature capsids. The current results show that in both infected- and uninfected-cell lysates, pUL28 coimmunoprecipitates with either pUL33 or pUL15, whereas pUL15 and pUL33 do not coimmunoprecipitate unless pUL28 is present. The UL28 protein was sufficient to stabilize pUL33 from proteasomal degradation in an engineered cell line and was necessary to stabilize pUL33 in infected cells, whereas pUL15 had no such effects. The presence of pUL33 was dispensable for the pUL15/pUL28 interaction in lysates of both infected and uninfected cells but augmented the tendency for pUL15 and pUL28 to coimmunoprecipitate. These data suggest that pUL28 and pUL33 interact directly and that pUL15 interacts directly with pUL28 but only indirectly with pUL33. It is logical to propose that the indirect interaction of pUL15 and pUL33 is mediated through the interaction of both proteins with pUL28. The data also suggest that one function of pUL33 is to optimize the pUL15/pUL28 interaction.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, C5143 Veterinary Education Center, Cornell University, Ithaca, NY 14850. Phone: (607) 253-3391. Fax: (607) 253-3384. E-mail: jdb11{at}cornell.edu.

Journal of Virology, June 2006, p. 5733-5739, Vol. 80, No. 12
0022-538X/06/$08.00+0     doi:10.1128/JVI.00125-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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