This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Perron, M. J. Articles by Sodroski, J. Search for Related Content PubMed PubMed Citation Articles by Perron, M. J. Articles by Sodroski, J.

 Previous Article  |  Next Article 

Journal of Virology, June 2006, p. 5631-5636, Vol. 80, No. 11
0022-538X/06/$08.00+0     doi:10.1128/JVI.00219-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Two Surface-Exposed Elements of the B30.2/SPRY Domain as Potency Determinants of N-Tropic Murine Leukemia Virus Restriction by Human TRIM5

Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Department of Pathology, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115,¹ Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115²

Received 31 January 2006/ Accepted 7 March 2006

Human TRIM5 (TRIM5_hu) potently restricts N-tropic (N-MLV), but not B-tropic, murine leukemia virus in a manner dependent upon residue 110 of the viral capsid. Rhesus monkey TRIM5 (TRIM5_rh) inhibits N-MLV only weakly. The study of human-monkey TRIM5 chimerae revealed that both the v1 and v3 variable regions of the B30.2/SPRY domain contain potency determinants for N-MLV restriction. These variable regions are predicted to be surface-exposed elements on one face of the B30.2 domain. Acidic residues in v3 complement basic residue 110 of the N-MLV capsid. The results support recognition of the retroviral capsid by the TRIM5 B30.2 domain.

This article has been cited by other articles:

• Wilson, S. J., Webb, B. L. J., Ylinen, L. M. J., Verschoor, E., Heeney, J. L., Towers, G. J. (2008). From the Cover: Independent evolution of an antiviral TRIMCyp in rhesus macaques. Proc. Natl. Acad. Sci. USA 105: 3557-3562 [Abstract] [Full Text]  
• James, L. C., Keeble, A. H., Khan, Z., Rhodes, D. A., Trowsdale, J. (2007). Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function. Proc. Natl. Acad. Sci. USA 104: 6200-6205 [Abstract] [Full Text]  
• Perron, M. J., Stremlau, M., Lee, M., Javanbakht, H., Song, B., Sodroski, J. (2007). The Human TRIM5{alpha} Restriction Factor Mediates Accelerated Uncoating of the N-Tropic Murine Leukemia Virus Capsid. J. Virol. 81: 2138-2148 [Abstract] [Full Text]