This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Antinone, S. E.
Right arrow Articles by Smith, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Antinone, S. E.
Right arrow Articles by Smith, G. A.

 Previous Article  |  Next Article 

Journal of Virology, June 2006, p. 5494-5498, Vol. 80, No. 11
0022-538X/06/$08.00+0     doi:10.1128/JVI.00026-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Herpesvirus Capsid Surface Protein, VP26, and the Majority of the Tegument Proteins Are Dispensable for Capsid Transport toward the Nucleus{dagger}

Sarah E. Antinone,1,{ddagger} George T. Shubeita,2,{ddagger} Kelly E. Coller,1 Joy I. Lee,1 Sarah Haverlock-Moyns,1 Steven P. Gross,2,§ and Gregory A. Smith1,§*

Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois,1 Department of Developmental and Cell Biology, University of California, Irvine, California2

Received 4 January 2006/ Accepted 6 March 2006

Upon entering a cell, alphaherpesvirus capsids are transported toward the minus ends of microtubules and ultimately deposit virus DNA within the host nucleus. The virus proteins that mediate this centripetal transport are unknown but are expected to be either viral tegument proteins, which are a group of capsid-associated proteins, or a surface component of the capsid itself. Starting with derivatives of pseudorabies virus that encode a fluorescent protein fused to a structural component of the virus, we have made a collection of 12 mutant viruses that lack either the VP26 capsid protein or an individual tegument protein. Using live-cell fluorescence microscopy, we tracked individual virus particles in axons following infection of primary sensory neurons. Quantitative analysis of the VP26-null virus indicates that this protein plays no observable role in capsid transport. Furthermore, viruses lacking tegument proteins that are nonessential for virus propagation in cell culture were also competent for axonal transport. These results indicate that a protein essential for viral propagation mediates transport of the capsid to the nucleus.

* Corresponding author. Mailing address: Department of Microbiology-Immunology, Ward, Rm. 10-105, Northwestern University Feinberg School of Medicine, Chicago, IL 60611. Phone: (312) 503-3745. Fax: (312) 503-1339. E-mail: g-smith3{at}northwestern.edu.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

{ddagger} S.E.A. and G.T.S. contributed equally to this work.

§ S.P.G. and G.A.S. are co-senior authors.

Journal of Virology, June 2006, p. 5494-5498, Vol. 80, No. 11
0022-538X/06/$08.00+0     doi:10.1128/JVI.00026-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McGraw, H. M., Friedman, H. M. (2009). Herpes Simplex Virus Type 1 Glycoprotein E Mediates Retrograde Spread from Epithelial Cells to Neurites. J. Virol. 83: 4791-4799 [Abstract] [Full Text]  
  • Krautwald, M., Fuchs, W., Klupp, B. G., Mettenleiter, T. C. (2009). Translocation of Incoming Pseudorabies Virus Capsids to the Cell Nucleus Is Delayed in the Absence of Tegument Protein pUL37. J. Virol. 83: 3389-3396 [Abstract] [Full Text]  
  • Lyman, M. G., Enquist, L. W. (2009). Herpesvirus Interactions with the Host Cytoskeleton. J. Virol. 83: 2058-2066 [Full Text]  
  • Krautwald, M., Maresch, C., Klupp, B. G., Fuchs, W., Mettenleiter, T. C. (2008). Deletion or green fluorescent protein tagging of the pUL35 capsid component of pseudorabies virus impairs virus replication in cell culture and neuroinvasion in mice. J. Gen. Virol. 89: 1346-1351 [Abstract] [Full Text]  
  • de Oliveira, A. P., Glauser, D. L., Laimbacher, A. S., Strasser, R., Schraner, E. M., Wild, P., Ziegler, U., Breakefield, X. O., Ackermann, M., Fraefel, C. (2008). Live Visualization of Herpes Simplex Virus Type 1 Compartment Dynamics. J. Virol. 82: 4974-4990 [Abstract] [Full Text]  
  • Coller, K. E., Lee, J. I-H., Ueda, A., Smith, G. A. (2007). The Capsid and Tegument of the Alphaherpesviruses Are Linked by an Interaction between the UL25 and VP1/2 Proteins. J. Virol. 81: 11790-11797 [Abstract] [Full Text]  
  • Dohner, K., Radtke, K., Schmidt, S., Sodeik, B. (2006). Eclipse Phase of Herpes Simplex Virus Type 1 Infection: Efficient Dynein-Mediated Capsid Transport without the Small Capsid Protein VP26.. J. Virol. 80: 8211-8224 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»