This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Teterina, N. L.
Right arrow Articles by Ehrenfeld, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Teterina, N. L.
Right arrow Articles by Ehrenfeld, E.

 Previous Article  |  Next Article 

Journal of Virology, June 2006, p. 5327-5337, Vol. 80, No. 11
0022-538X/06/$08.00+0     doi:10.1128/JVI.02684-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evidence for Functional Protein Interactions Required for Poliovirus RNA Replication

Natalya L. Teterina,1 Eric Levenson,1 Mario S. Rinaudo,1 Denise Egger,2 Kurt Bienz,2 Alexander E. Gorbalenya,3 and Ellie Ehrenfeld1*

Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, Maryland 20892,1 Institute for Medical Microbiology, University of Basel, Basel, Switzerland,2 Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands3

Received 21 December 2005/ Accepted 8 March 2006

Poliovirus protein 2C contains a predicted N-terminal amphipathic helix that mediates association of the protein with the membranes of the viral RNA replication complex. A chimeric virus that contains sequences encoding the 18-residue core from the orthologous amphipathic helix from human rhinovirus type 14 (HRV14) was constructed. The chimeric virus exhibited defects in viral RNA replication and produced minute plaques on HeLa cell monolayers. Large plaque variants that contained mutations within the 2C-encoding region were generated upon subsequent passage. However, the majority of viruses that emerged with improved growth properties contained no changes in the region encoding 2C. Sequence analysis and reconstruction of genomes with individual mutations revealed changes in 3A or 2B sequences that compensated for the HRV14 amphipathic helix in the polio 2C-containing proteins, implying functional interactions among these proteins during the replication process. Direct binding between these viral proteins was confirmed by mammalian cell two-hybrid analysis.

* Corresponding author. Mailing address: LID, NIAID, NIH, Bldg. 50, Room 6120, 50 South Drive, Bethesda, MD 20892-8011. Phone: (301) 594-1654. Fax: (301) 435-6021. E-mail: eehrenfeld{at}niaid.nih.gov.

Journal of Virology, June 2006, p. 5327-5337, Vol. 80, No. 11
0022-538X/06/$08.00+0     doi:10.1128/JVI.02684-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • De Palma, A. M., Thibaut, H. J., van der Linden, L., Lanke, K., Heggermont, W., Ireland, S., Andrews, R., Arimilli, M., Al-Tel, T. H., De Clercq, E., van Kuppeveld, F., Neyts, J. (2009). Mutations in the Nonstructural Protein 3A Confer Resistance to the Novel Enterovirus Replication Inhibitor TTP-8307. Antimicrob. Agents Chemother. 53: 1850-1857 [Abstract] [Full Text]  
  • Cordey, S., Gerlach, D., Junier, T., Zdobnov, E. M., Kaiser, L., Tapparel, C. (2008). The cis-acting replication elements define human enterovirus and rhinovirus species. RNA 14: 1568-1578 [Abstract] [Full Text]  
  • Belsham, G. J., Normann, P. (2008). Dynamics of picornavirus RNA replication within infected cells. J. Gen. Virol. 89: 485-493 [Abstract] [Full Text]  
  • Yin, J., Liu, Y., Wimmer, E., Paul, A. V. (2007). Complete protein linkage map between the P2 and P3 non-structural proteins of poliovirus. J. Gen. Virol. 88: 2259-2267 [Abstract] [Full Text]  
  • Moffat, K., Knox, C., Howell, G., Clark, S. J., Yang, H., Belsham, G. J., Ryan, M., Wileman, T. (2007). Inhibition of the Secretory Pathway by Foot-and-Mouth Disease Virus 2BC Protein Is Reproduced by Coexpression of 2B with 2C, and the Site of Inhibition Is Determined by the Subcellular Location of 2C. J. Virol. 81: 1129-1139 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»