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Journal of Virology, May 2006, p. 5086-5091, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.5086-5091.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Chaperone-Mediated In Vitro Disassembly of Polyoma- and Papillomaviruses

Laura R. Chromy,{dagger} Amy Oltman, Patricia A. Estes, and Robert L. Garcea*

Section of Pediatric Hematology/Oncology and Molecular Biology Program, University of Colorado Health Sciences Center, Aurora, Colorado 80045

Received 15 November 2005/ Accepted 20 February 2006

Hsp70 chaperones play a role in polyoma- and papillomavirus assembly, as evidenced by their interaction in vivo with polyomavirus capsid proteins at late times after virus infection and by their ability to assemble viral capsomeres into capsids in vitro. We studied whether Hsp70 chaperones might also participate in the uncoating reaction. In vivo, Hsp70 coimmunoprecipitated with polyomavirus virion VP1 at 3 h after infection of mouse cells. In vitro, prokaryotic and eukaryotic Hsp70 chaperones efficiently disassembled polyoma- and papillomavirus-like particles and virions in energy-dependent reactions. These observations support a role for cell chaperones in the disassembly of these viruses.

* Corresponding author. Mailing address: UCDHSC at Fitzsimons, Pediatrics, Mail Stop 8302, P.O. Box 6511, Aurora, CO 80045. Phone: (303) 724-4000. Fax: (303) 724-4015. E-mail: bob.garcea{at}uchsc.edu.

{dagger} Present address: University of Michigan School of Medicine, 1301 Catherine Street, Ann Arbor, MI 48109-0611.

Journal of Virology, May 2006, p. 5086-5091, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.5086-5091.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Conway, M.J., Meyers, C. (2009). Replication and Assembly of Human Papillomaviruses. JDR 88: 307-317 [Abstract] [Full Text]  
  • Bird, G., O'Donnell, M., Moroianu, J., Garcea, R. L. (2008). Possible Role for Cellular Karyopherins in Regulating Polyomavirus and Papillomavirus Capsid Assembly. J. Virol. 82: 9848-9857 [Abstract] [Full Text]  
  • Ivanovic, T., Agosto, M. A., Chandran, K., Nibert, M. L. (2007). A Role for Molecular Chaperone Hsc70 in Reovirus Outer Capsid Disassembly. J. Biol. Chem. 282: 12210-12219 [Abstract] [Full Text]  


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