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Journal of Virology, May 2006, p. 5078-5081, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.5078-5081.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Epstein-Barr Virus BMRF1 Gene Is Essential for Lytic Virus Replication

Bernhard Neuhierl and Henri-Jacques Delecluse*

German Cancer Research Center, Department of Virus Associated Tumours, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany

Received 21 October 2005/ Accepted 20 February 2006

The Epstein-Barr virus (EBV) BMRF1 protein is a DNA polymerase processivity factor. We have deleted the BMRF1 open reading frame from the EBV genome and assessed the {Delta}BMRF1 EBV phenotype. {Delta}BMRF1 viruses were replication deficient, but the wild-type phenotype could be restored by BMRF1 trans-complementation. The replication-deficient phenotype included impaired lytic DNA replication and late protein expression. {Delta}BMRF1 and wild-type viruses were undistinguishable in terms of their ability to transform primary B cells. Our results provide genetic evidence that BMRF1 is essential for lytic replication of the EBV genome.

* Corresponding author. Mailing address: German Cancer Research Center, Department of Virus Associated Tumours, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany. Phone: 49 6221 424870. Fax: 49 6221 424852. E-mail: h.delecluse{at}dkfz.de.

Journal of Virology, May 2006, p. 5078-5081, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.5078-5081.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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