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Journal of Virology, May 2006, p. 5059-5064, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.5059-5064.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Double-Stranded RNA Is Produced by Positive-Strand RNA Viruses and DNA Viruses but Not in Detectable Amounts by Negative-Strand RNA Viruses

Friedemann Weber,1* Valentina Wagner,1 Simon B. Rasmussen,2 Rune Hartmann,3 and Søren R. Paludan2

Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, D-79008 Freiburg, Germany,1 Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus, Denmark,2 Department of Molecular Biology, University of Aarhus, DK-8000 Aarhus, Denmark3

Received 26 October 2005/ Accepted 23 February 2006

Double-stranded RNA (dsRNA) longer than 30 bp is a key activator of the innate immune response against viral infections. It is widely assumed that the generation of dsRNA during genome replication is a trait shared by all viruses. However, to our knowledge, no study exists in which the production of dsRNA by different viruses is systematically investigated. Here, we investigated the presence and localization of dsRNA in cells infected with a range of viruses, employing a dsRNA-specific antibody for immunofluorescence analysis. Our data revealed that, as predicted, significant amounts of dsRNA can be detected for viruses with a genome consisting of positive-strand RNA, dsRNA, or DNA. Surprisingly, however, no dsRNA signals were detected for negative-strand RNA viruses. Thus, dsRNA is indeed a general feature of most virus groups, but negative-strand RNA viruses appear to be an exception to that rule.

* Corresponding author. Mailing address: Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, D-79008 Freiburg, Germany. Phone: 49-761-203-6614. Fax: 49-761-203-6562. E-mail: friedemann.weber{at}uniklinik-freiburg.de.

Journal of Virology, May 2006, p. 5059-5064, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.5059-5064.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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