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Journal of Virology, May 2006, p. 4998-5009, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.4998-5009.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular and Biological Characterization of Deformed Wing Virus of Honeybees (Apis mellifera L.)

Gaetana Lanzi,1,{dagger} Joachim R. de Miranda,2,{ddagger} Maria Beatrice Boniotti,1 Craig E. Cameron,3 Antonio Lavazza,1 Lorenzo Capucci,1 Scott M. Camazine,2,§ and Cesare Rossi1*

Reparto di Biologia Molecolare, Istituto Zooprofilattico Sperimentale Lombardia e Emilia-Romagna, via Bianchi 9, Brescia, Italy 25124-I,1 Department of Entomology,2 Department of Biochemistry, Penn State University, University Park, Pennsylvania 168023

Received 16 November 2005/ Accepted 21 February 2006

Deformed wing virus (DWV) of honeybees (Apis mellifera) is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult bees. The virus was purified from diseased insects, and its genome was cloned and sequenced. The genomic RNA of DWV is 10,140 nucleotides in length and contains a single large open reading frame encoding a 328-kDa polyprotein. The coding sequence is flanked by a 1,144-nucleotide 5' nontranslated leader sequence and a 317-nucleotide 3' nontranslated region, followed by a poly(A) tail. The three major structural proteins, VP1 (44 kDa), VP2 (32 kDa), and VP3 (28 kDa), were identified, and their genes were mapped to the N-terminal section of the polyprotein. The C-terminal part of the polyprotein contains sequence motifs typical of well-characterized picornavirus nonstructural proteins: an RNA helicase, a chymotrypsin-like 3C protease, and an RNA-dependent RNA polymerase. The genome organization, capsid morphology, and sequence comparison data indicate that DWV is a member of the recently established genus Iflavirus.

* Corresponding author. Present address: Policlinico S. Orsola-Malpighi, PAD 11 Unit of Medical Genetics, via Massarenti 9, Bologna 40137, Italy. Phone: 39 051 4294726. Fax: 39 051 6364004. E-mail: cesare.rossi{at}med.unibo.it.

{dagger} Present address: Institute of Molecular Medicine A. Nocivelli and Pediatric Clinic, University of Brescia, Brescia, Italy.

{ddagger} Present address: School of Biology, Queens University Belfast, 97 Lisburn Rd., Belfast BT9 7BL, Northern Ireland.

§ Present address: Altoona Hospital, 620 Howard Ave., Altoona, PA 16601.

Journal of Virology, May 2006, p. 4998-5009, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.4998-5009.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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