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Expression of m157, a Murine Cytomegalovirus-Encoded Putative Major Histocompatibility Class I (MHC-I)-Like Protein, Is Independent of Viral Regulation of Host MHC-I

Sandeep K. Tripathy,¹ Hamish R. C. Smith,^2, Erika A. Holroyd,² Jeanette T. Pingel,² and Wayne M. Yokoyama^2*

Gastroenterology Division, Department of Medicine,¹ Rheumatology Division, Department of Medicine, Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, Missouri 63110²

Received 11 August 2005/ Accepted 13 October 2005

A murine cytomegalovirus (MCMV)-encoded protein, m157, has a putative major histocompatibility complex class I (MHC-I) structure and is recognized by the Ly49H NK cell activation receptor. Using a monoclonal antibody against m157, in this study we directly demonstrated that m157 is a cell surface-expressed glycophosphatidylinositol-anchored protein with early viral gene kinetics. Beta-2 microglobulin and TAP1 (transporter associated with antigen processing 1) were not required for its expression. MCMV-encoded proteins that down-regulate MHC-I did not affect the expression of m157. Thus, m157 is expressed on infected cells in a manner independent of viral regulation of host MHC-I.

Present address: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305.

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