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 Previous Article

Journal of Virology, January 2006, p. 545-550, Vol. 80, No. 1
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.1.545-550.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Expression of m157, a Murine Cytomegalovirus-Encoded Putative Major Histocompatibility Class I (MHC-I)-Like Protein, Is Independent of Viral Regulation of Host MHC-I

Sandeep K. Tripathy,1 Hamish R. C. Smith,2,{dagger} Erika A. Holroyd,2 Jeanette T. Pingel,2 and Wayne M. Yokoyama2*

Gastroenterology Division, Department of Medicine,1 Rheumatology Division, Department of Medicine, Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, Missouri 631102

Received 11 August 2005/ Accepted 13 October 2005

A murine cytomegalovirus (MCMV)-encoded protein, m157, has a putative major histocompatibility complex class I (MHC-I) structure and is recognized by the Ly49H NK cell activation receptor. Using a monoclonal antibody against m157, in this study we directly demonstrated that m157 is a cell surface-expressed glycophosphatidylinositol-anchored protein with early viral gene kinetics. Beta-2 microglobulin and TAP1 (transporter associated with antigen processing 1) were not required for its expression. MCMV-encoded proteins that down-regulate MHC-I did not affect the expression of m157. Thus, m157 is expressed on infected cells in a manner independent of viral regulation of host MHC-I.

* Corresponding author. Mailing address: Washington University School of Medicine, Rheumatology, Box 8045, 660 S. Euclid Ave, St. Louis, MO 63110. Phone: (314) 362-9075. Fax: (314) 362-9257. E-mail: yokoyama{at}im.wustl.edu.

{dagger} Present address: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305.

Journal of Virology, January 2006, p. 545-550, Vol. 80, No. 1
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.1.545-550.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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