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J Virol. 1971 November; 8(5): 766-770
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Structural Proteins of Adenovirus-Associated Viruses

National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461

ABSTRACT

The structural proteins of adenovirus-associated virus (AAV) types 1, 2, and 3 were analyzed by acrylamide gel electrophoresis. In each case, one major protein (C) and two minor proteins (A and B) were identified. Component C had an estimated molecular weight of 62,000 daltons, and the molecular weights of components A and B were found to be 87,000 and 73,000 daltons, respectively. Coelectrophoresis of adenovirus and AAV proteins revealed an overlap only between the adenovirus fiber-penton component and the AAV C polypeptide. Among AAV serotypes, homologous components were electrophoretically identical, except that the C component of AAV-2 was of slightly lower molecular weight than the C components of AAV-1 and AAV-3. The relative incorporation of ¹⁴C-arginine and ¹⁴C-mixed amino acids into the three polypeptides of AAV-2 was similar, indicating an absence of an arginine-rich component. In addition, AAV-2 was found to have a substantially lower arginine content than helper adenoviruses.

¹ Recipient of American Cancer Society faculty award PRA-69.

² Present address: Roche Institute of Molecular Biology, Nutley, N.J.

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