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Quantitative Binding of ¹²⁵I-Concanavalin A to Normal and Transformed Cells

Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305

ABSTRACT

We have measured the quantitative binding of the radioactively labeled agglutinin ¹²⁵I-concanavalin A to normal mammalian cells and simian virus 40- and polyoma virus-transformed cells from tissue culture. Parallel measurements of the amount of ¹²⁵I-concanavalin A necessary to cause agglutination of the cells in suspension were carried out. The transformed and nontransformed cells used for these experiments show large differences in their ability to be agglutinated by ¹²⁵I-concanavalin A. However, these cell lines have the same number of specific binding sites and similar affinities for the agglutinin whether transformed, trypsinized, or nontransformed. We conclude that the differential capacity of concanavalin A to agglutinate transformed cells relative to normal cells does not result from differences in the number of binding sites between the two types of cells.

¹ Fellow of the Jane Coffin Childs Memorial Fund for Medical Research. Present address: Abteilung für Molekulare Biologie, Max-Planck-Institut für Biophysikalische Chemie, D-3400 Goettingen, West Germany.

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