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Effect of Pactamycin on Synthesis of Poliovirus Proteins: a Method for Genetic Mapping

Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

ABSTRACT

We have studied the effect of the drug pactamycin on protein synthesis in poliovirus-infected HeLa cells. At a concentration which primarily inhibits initiation of protein synthesis, the spectrum of poliovirus proteins synthesized is markedly changed. The amount of NCVP 1, the capsid precursor, is greatly reduced relative to NCVP 2 and the amount of NCVP X is slightly reduced. Since it is believed that there is only one major site for the initiation of protein synthesis on the poliovirus genome, we interpret this differential effect on the poliovirus proteins to be an indication of their relative distance from the initiation site. On this basis, we propose a gene order for the poliovirus genome (5' 3') of NCVP 1, NCVP X, NCVP 2.