This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Engelberg, H. Articles by Soudry, E. Search for Related Content PubMed PubMed Citation Articles by Engelberg, H. Articles by Soudry, E.

Ribonucleic Acid Bacteriophage Release: Requirement for Host-Controlled Protein Synthesis

Department of Molecular Biology, Institute of Microbiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel

ABSTRACT

The release of the ribonucleic acid (RNA)-containing phage MS2 from Escherichia coli is accompanied by cellular lysis at 37 C, whereas at 30 C phage are released from intact cells. Chloramphenicol or rifampin prevents the release of progeny phage particles at both temperatures. Neither drug causes an immediate cessation of phage release and after inhibition of protein synthesis by chloramphenicol phage release proceeds for about 17 min at 37 C and about 35 min at 30 C. Rifampin does not inhibit phage release from mutant cells possessing a rifampin-resistant deoxyribonucleic acid-dependent RNA polymerase. The results indicate that a short-lived host-controlled protein(s) is essential for the release of RNA phage particles at both temperatures.

This article has been cited by other articles:

• Shtatland, T., Gill, S. C., Javornik, B. E., Johansson, H. E., Singer, B. S., Uhlenbeck, O. C., Zichi, D. A., Gold, L. (2000). Interactions of Escherichia coli RNA with bacteriophage MS2 coat protein: genomic SELEX. Nucleic Acids Res 28: e93-e93 [Abstract] [Full Text]