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J Virol. 1971 August; 8(2): 215-224
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses IV. Characterization of the Simian Virus 40 Ribonucleic Acid Species Induced by Wild-Type Simian Virus 40 and by the Nondefective Hybrid Virus, Ad2⁺ND₁

Research Division of Infectious Diseases, Children's Hospital Medical Center and Departments of Medicine and Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
Medicine Branch, National Cancer Institute, and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
Department of Medicine, University of Missouri, Columbia, Missouri 65201

ABSTRACT

Ad2⁺ND₁, a nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid virus, has been previously shown to contain a small segment of the SV40 genome covalently linked to Ad2 deoxyribonucleic acid (DNA). The SV40 portion of this hybrid virus has been characterized by relating the SV40-specific ribonucleic acid (RNA) sequences transcribed from the Ad2⁺ND₁ DNA to those transcribed from the DNA of SV40 itself. RNA-DNA hybridization-competition studies indicate that the SV40 component of Ad2⁺ND₁ consists of some, but not all, of that part of the SV40 genome which is transcribed early, i.e., prior to viral DNA replication, in SV40 lytic infection.