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Influence of Polycations on the Interaction Between Poliovirus Multistranded Ribonucleic Acid and HeLa Cells ¹

^a Institut für Mikrobiologie, Medizinische Hochschule, Hannover, Germany
Department of Cell Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110

ABSTRACT

In contrast to single-stranded viral ribonucleic acid (RNA), poliovirus multistranded RNA (RI-RNA) is poorly adsorbed to suspended HeLa cells in the absence of polycations. In the presence of 20 µg of diethylaminoethyl (DEAE) dextran per ml and other polycations, 90% or more of the RI-RNA is adsorbed to HeLa cells within 2 min at 37 C. However, only 30% of the RI-RNA label penetrates into the cells independent of the concentration of DEAE dextran applied. DEAE dextran is adsorbed almost quantitatively to HeLa cells within 3 min at 37 C. Most of the DEAE dextran remains bound to the cell membrane and available for binding of RI-RNA for 15 min at 37 C.

¹ This work was submitted by P.W. to the Medizinische Hochschule Hannover, Germany, in partial fulfillment of the requirements for the Degree of Doctor of Medicine.