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Requirement of Protein Synthesis for Bacteriophage X174 Superinfection Exclusion

^a Division of Biology, California Institute of Technology, Pasadena, California 91109

ABSTRACT

Within 5 to 10 min at 37 C, bacteria infected by bacteriophage X174 acquire the ability to exclude superinfecting X particles from reproducing themselves. The superinfecting phage are blocked at a stage prior to synthesis of the parental replicative form molecule; the superinfecting deoxyribonucleic acid remains as intact (infective) single strands. Establishment of superinfection exclusion and its maintenance require protein synthesis.

¹ Present address: Department of Bacteriology and Immunology, School of Medicine, University of North Carolina, Chapel Hill, N.C. 27514.

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