This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Engelhardt, O. G.
Right arrow Articles by Fodor, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Engelhardt, O. G.
Right arrow Articles by Fodor, E.

 Previous Article  |  Next Article 

Journal of Virology, May 2005, p. 5812-5818, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5812-5818.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Association of the Influenza A Virus RNA-Dependent RNA Polymerase with Cellular RNA Polymerase II

Othmar G. Engelhardt, Matt Smith, and Ervin Fodor*

Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom

Received 6 October 2004/ Accepted 3 December 2004

Transcription by the influenza virus RNA-dependent RNA polymerase is dependent on cellular RNA processing activities that are known to be associated with cellular RNA polymerase II (Pol II) transcription, namely, capping and splicing. Therefore, it had been hypothesized that transcription by the viral RNA polymerase and Pol II might be functionally linked. Here, we demonstrate for the first time that the influenza virus RNA polymerase complex interacts with the large subunit of Pol II via its C-terminal domain. The viral polymerase binds hyperphosphorylated forms of Pol II, indicating that it targets actively transcribing Pol II. In addition, immunofluorescence analysis is consistent with a new model showing that influenza virus polymerase accumulates at Pol II transcription sites. The present findings provide a framework for further studies to elucidate the mechanistic principles of transcription by a viral RNA polymerase and have implications for the regulation of Pol II activities in infected cells.

* Corresponding author. Mailing address: Sir William Dunn School of Pathology, University of Oxford, South Parks Rd., Oxford OX1 3RE, United Kingdom. Phone: 44-(0)1865-275580. Fax: 44-(0)1865-275556. E-mail: ervin.fodor{at}path.ox.ac.uk.

Journal of Virology, May 2005, p. 5812-5818, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5812-5818.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rodriguez, A., Perez-Gonzalez, A., Hossain, M. J., Chen, L.-M., Rolling, T., Perez-Brena, P., Donis, R., Kochs, G., Nieto, A. (2009). Attenuated Strains of Influenza A Viruses Do Not Induce Degradation of RNA Polymerase II. J. Virol. 83: 11166-11174 [Abstract] [Full Text]  
  • Wang, P., Song, W., Mok, B. W.-Y., Zhao, P., Qin, K., Lai, A., Smith, G. J. D., Zhang, J., Lin, T., Guan, Y., Chen, H. (2009). Nuclear Factor 90 Negatively Regulates Influenza Virus Replication by Interacting with Viral Nucleoprotein. J. Virol. 83: 7850-7861 [Abstract] [Full Text]  
  • Rolling, T., Koerner, I., Zimmermann, P., Holz, K., Haller, O., Staeheli, P., Kochs, G. (2009). Adaptive Mutations Resulting in Enhanced Polymerase Activity Contribute to High Virulence of Influenza A Virus in Mice. J. Virol. 83: 6673-6680 [Abstract] [Full Text]  
  • Robb, N. C., Smith, M., Vreede, F. T., Fodor, E. (2009). NS2/NEP protein regulates transcription and replication of the influenza virus RNA genome. J. Gen. Virol. 90: 1398-1407 [Abstract] [Full Text]  
  • Kuo, R.-L., Krug, R. M. (2009). Influenza A Virus Polymerase Is an Integral Component of the CPSF30-NS1A Protein Complex in Infected Cells. J. Virol. 83: 1611-1616 [Abstract] [Full Text]  
  • Rameix-Welti, M.-A., Tomoiu, A., Dos Santos Afonso, E., van der Werf, S., Naffakh, N. (2009). Avian Influenza A Virus Polymerase Association with Nucleoprotein, but Not Polymerase Assembly, Is Impaired in Human Cells during the Course of Infection. J. Virol. 83: 1320-1331 [Abstract] [Full Text]  
  • Gabriel, G., Abram, M., Keiner, B., Wagner, R., Klenk, H.-D., Stech, J. (2007). Differential Polymerase Activity in Avian and Mammalian Cells Determines Host Range of Influenza Virus. J. Virol. 81: 9601-9604 [Abstract] [Full Text]  
  • Garaigorta, U., Ortin, J. (2007). Mutation analysis of a recombinant NS replicon shows that influenza virus NS1 protein blocks the splicing and nucleo-cytoplasmic transport of its own viral mRNA. Nucleic Acids Res 35: 4573-4582 [Abstract] [Full Text]  
  • Rodriguez, A., Perez-Gonzalez, A., Nieto, A. (2007). Influenza Virus Infection Causes Specific Degradation of the Largest Subunit of Cellular RNA Polymerase II. J. Virol. 81: 5315-5324 [Abstract] [Full Text]  
  • Deng, T., Engelhardt, O. G., Thomas, B., Akoulitchev, A. V., Brownlee, G. G., Fodor, E. (2006). Role of Ran Binding Protein 5 in Nuclear Import and Assembly of the Influenza Virus RNA Polymerase Complex. J. Virol. 80: 11911-11919 [Abstract] [Full Text]  
  • Hara, K., Schmidt, F. I., Crow, M., Brownlee, G. G. (2006). Amino Acid Residues in the N-Terminal Region of the PA Subunit of Influenza A Virus RNA Polymerase Play a Critical Role in Protein Stability, Endonuclease Activity, Cap Binding, and Virion RNA Promoter Binding.. J. Virol. 80: 7789-7798 [Abstract] [Full Text]  
  • Martin, A., Staeheli, P., Schneider, U. (2006). RNA Polymerase II-Controlled Expression of Antigenomic RNA Enhances the Rescue Efficacies of Two Different Members of the Mononegavirales Independently of the Site of Viral Genome Replication.. J. Virol. 80: 5708-5715 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»