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Journal of Virology, May 2005, p. 5577-5584, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5577-5584.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Novel Macula-Like Virus Identified in Bombyx mori Cultured Cells

Susumu Katsuma,1 Shinichiro Tanaka,1 Naoko Omuro,1 Lisa Takabuchi,1 Takaaki Daimon,1 Shigeo Imanishi,2 Shuichi Yamashita,1 Masashi Iwanaga,1,{dagger} Kazuei Mita,2 Susumu Maeda,3,{ddagger} Masahiko Kobayashi,1 and Toru Shimada1*

Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo,1 National Institute of Agrobiological Sciences, Tsukuba, Ibaraki,2 Laboratory of Molecular Entomology and Baculovirology, RIKEN, Wako, Saitama, Japan3

Received 1 September 2004/ Accepted 30 November 2004

We identified a novel, 6,513-bp-long RNA, termed Bombyx mori macula-like latent virus (BmMLV) RNA, which abundantly expressed in B. mori cultured BmN cells. BmMLV RNA potentially encodes two proteins, putative RNA replicase and coat protein, which share structural features and sequence similarities with those of a plant RNA virus, the genus Maculavirus. Northern blot analysis showed that two transcripts were expressed in BmN cells: a 6.5-kb-long RNA, which contains both putative RNA replicase and coat protein genes, and a 1.2-kb-long RNA, which contains only a coat protein gene. Southern blot analysis showed that BmMLV RNA is not carried by the B. mori genome. RT-PCR analysis also revealed the presence of BmMLV RNA in several B. mori cell lines other than BmN cells, suggesting that BmMLV RNA latently exists in B. mori cultured cells. Infection studies showed that BmMLV virions were able to infect BmMLV-negative Spodoptera frugiperda Sf-9 cells and B. mori larvae. Electron microscopy and Northern blot analysis of a purified BmMLV revealed that isometric virions appear to be 28 to 30 nm in diameter and contain a 6.5-kb genomic RNA. These results showed that BmMLV is a novel macula-like virus infectious to and replicable in B. mori-derived cells.

* Corresponding author. Mailing address: Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan. Phone: 81-3-5841-8011. Fax: 81-3-5841-8130. E-mail: shimada{at}ss.ab.a.u-tokyo.ac.jp.

{dagger} Present address: New Frontiers Research Laboratory, Toray Industries, Inc., Kamakura, Kanagawa 248-8555, Japan.

{ddagger} Deceased.

Journal of Virology, May 2005, p. 5577-5584, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5577-5584.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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