This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burdeinick-Kerr, R.
Right arrow Articles by Griffin, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burdeinick-Kerr, R.
Right arrow Articles by Griffin, D. E.

 Previous Article  |  Next Article 

Journal of Virology, May 2005, p. 5374-5385, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5374-5385.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Gamma Interferon-Dependent, Noncytolytic Clearance of Sindbis Virus Infection from Neurons In Vitro

Rebeca Burdeinick-Kerr and Diane E. Griffin*

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205

Received 22 July 2004/ Accepted 30 December 2004

Due to the nonrenewable nature of neurons, recovery from viral infection of the central nervous system requires noncytopathic mechanisms for control of virus replication. Recovery from alphavirus encephalitis can occur without apparent neurological damage through the effects of antibody and gamma interferon (IFN-{gamma}). To establish an in vitro cell culture system that will allow the study of mechanisms of IFN-{gamma}-mediated control of Sindbis virus (SINV) replication in neurons, we have characterized the susceptibility to SINV infection and IFN-{gamma} responsiveness of two neuronal cell lines that can be differentiated in vitro: CSM14.1, a rat nigral cell line, and NSC34, a mouse motor neuron cell line. Undifferentiated CSM14.1 and NSC34 cells were permissive for SINV and susceptible to virus-induced cell death. With differentiation, CSM14.1 cells reduced virus replication and became progressively resistant to virus-induced cell death, resulting in prolonged virus replication. NSC34 cells did not differentiate completely and became only partially resistant to SINV infection. Both CSM14.1 and NSC34 cells responded to pretreatment with IFN-{gamma} by decreasing SINV replication. Differentiated CSM14.1 cells treated 24 h after infection with IFN-{gamma} responded with increased cell viability and clearance of infectious virus. IFN-{gamma} treatment sequentially altered the ratio of genomic to subgenomic viral RNA synthesis, promoted recovery of cellular protein synthesis, reduced viral protein synthesis, and inhibited viral RNA transcription within 24 h after treatment. We conclude that CSM14.1 cells provide an excellent model for the study of IFN-{gamma}-mediated noncytolytic clearance of SINV from mature neurons.

* Corresponding author. Mailing address: W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, Maryland 21205. Phone: (410) 955-3459. Fax: (410) 955-0105. E-mail:dgriffin{at}jhsph.edu.

Journal of Virology, May 2005, p. 5374-5385, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5374-5385.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Burdeinick-Kerr, R., Govindarajan, D., Griffin, D. E. (2009). Noncytolytic Clearance of Sindbis Virus Infection from Neurons by Gamma Interferon Is Dependent on Jak/Stat Signaling. J. Virol. 83: 3429-3435 [Abstract] [Full Text]  
  • Brien, J. D., Uhrlaub, J. L., Nikolich-Zugich, J. (2008). West Nile Virus-Specific CD4 T Cells Exhibit Direct Antiviral Cytokine Secretion and Cytotoxicity and Are Sufficient for Antiviral Protection. J. Immunol. 181: 8568-8575 [Abstract] [Full Text]  
  • Ng, C. G., Coppens, I., Govindarajan, D., Pisciotta, J., Shulaev, V., Griffin, D. E. (2008). Effect of host cell lipid metabolism on alphavirus replication, virion morphogenesis, and infectivity. Proc. Natl. Acad. Sci. USA 105: 16326-16331 [Abstract] [Full Text]  
  • Pedras-Vasconcelos, J. A., Puig, M., Sauder, C., Wolbert, C., Ovanesov, M., Goucher, D., Verthelyi, D. (2008). Immunotherapy with CpG Oligonucleotides and Antibodies to TNF-{alpha} Rescues Neonatal Mice from Lethal Arenavirus-Induced Meningoencephalitis. J. Immunol. 180: 8231-8240 [Abstract] [Full Text]  
  • Greene, I. P., Lee, E.-Y., Prow, N., Ngwang, B., Griffin, D. E. (2008). Protection from fatal viral encephalomyelitis: AMPA receptor antagonists have a direct effect on the inflammatory response to infection. Proc. Natl. Acad. Sci. USA 105: 3575-3580 [Abstract] [Full Text]  
  • Burdeinick-Kerr, R., Wind, J., Griffin, D. E. (2007). Synergistic Roles of Antibody and Interferon in Noncytolytic Clearance of Sindbis Virus from Different Regions of the Central Nervous System. J. Virol. 81: 5628-5636 [Abstract] [Full Text]  
  • Samuel, M. A., Diamond, M. S. (2006). Pathogenesis of West Nile Virus Infection: a Balance between Virulence, Innate and Adaptive Immunity, and Viral Evasion. J. Virol. 80: 9349-9360 [Full Text]  
  • Shrestha, B., Wang, T., Samuel, M. A., Whitby, K., Craft, J., Fikrig, E., Diamond, M. S. (2006). Gamma Interferon Plays a Crucial Early Antiviral Role in Protection against West Nile Virus Infection.. J. Virol. 80: 5338-5348 [Abstract] [Full Text]  
  • Brzozka, K., Finke, S., Conzelmann, K.-K. (2006). Inhibition of Interferon Signaling by Rabies Virus Phosphoprotein P: Activation-Dependent Binding of STAT1 and STAT2. J. Virol. 80: 2675-2683 [Abstract] [Full Text]  
  • Shrestha, B., Samuel, M. A., Diamond, M. S. (2006). CD8+ T Cells Require Perforin To Clear West Nile Virus from Infected Neurons. J. Virol. 80: 119-129 [Abstract] [Full Text]  
  • Samuel, M. A., Diamond, M. S. (2005). Alpha/Beta Interferon Protects against Lethal West Nile Virus Infection by Restricting Cellular Tropism and Enhancing Neuronal Survival. J. Virol. 79: 13350-13361 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»