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Journal of Virology, May 2005, p. 5304-5314, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5304-5314.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Complete Genome Sequence and In Planta Subcellular Localization of Maize Fine Streak Virus Proteins

Chi-Wei Tsai,1 Margaret G. Redinbaugh,2,3 Kristen J. Willie,3 Sharon Reed,1 Michael Goodin,4 and Saskia A. Hogenhout1*

Department of Entomology,1 Department of Plant Pathology, Ohio Agricultural Research and Development Center, The Ohio State University,2 U.S. Department of Agriculture, Wooster, Ohio,3 Department of Plant Pathology, University of Kentucky, Lexington, Kentucky4

Received 3 September 2004/ Accepted 9 December 2004

The genome of the nucleorhabdovirus maize fine streak virus (MFSV) consists of 13,782 nucleotides of nonsegmented, negative-sense, single-stranded RNA. The antigenomic strand consisted of seven open reading frames (ORFs), and transcripts of all ORFs were detected in infected plants. ORF1, ORF6, and ORF7 had significant similarities to the nucleocapsid protein (N), glycoprotein (G), and polymerase (L) genes of other rhabdoviruses, respectively, whereas the ORF2, ORF3, ORF4, and ORF5 proteins had no significant similarities. The N (ORF1), ORF4, and ORF5 proteins localized to nuclei, consistent with the presence of nuclear localization signals (NLSs) in these proteins. ORF5 likely encodes the matrix protein (M), based on its size, the position of its NLS, and the localization of fluorescent protein fusions to the nucleus. ORF2 probably encodes the phosphoprotein (P) because, like the P protein of Sonchus yellow net virus (SYNV), it was spread throughout the cell when expressed alone but was relocalized to a subnuclear locus when coexpressed with the MFSV N protein. Unexpectedly, coexpression of the MFSV N and P proteins, but not the orthologous proteins of SYNV, resulted in accumulations of both proteins in the nucleolus. The N and P protein relocalization was specific to cognate proteins of each virus. The subcellular localizations of the MFSV ORF3 and ORF4 proteins were distinct from that of the SYNV sc4 protein, suggesting different functions. To our knowledge, this is the first comparative study of the cellular localizations of plant rhabdoviral proteins. This study indicated that plant rhabdoviruses are diverse in genome sequence and viral protein interactions.

* Corresponding author. Mailing address: Department of Entomology, The Ohio State University-Ohio Agricultural Research and Development Center (OARDC), 1680 Madison Ave., Wooster, OH 44691. Phone: (330) 263-3730. Fax: (330) 263-3686. E-mail: hogenhout.1{at}osu.edu.

Journal of Virology, May 2005, p. 5304-5314, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5304-5314.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Goodin, M. M., Chakrabarty, R., Yelton, S., Martin, K., Clark, A., Brooks, R. (2007). Membrane and protein dynamics in live plant nuclei infected with Sonchus yellow net virus, a plant-adapted rhabdovirus. J. Gen. Virol. 88: 1810-1820 [Abstract] [Full Text]  
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  • Kondo, H., Maeda, T., Shirako, Y., Tamada, T. (2006). Orchid fleck virus is a rhabdovirus with an unusual bipartite genome.. J. Gen. Virol. 87: 2413-2421 [Abstract] [Full Text]  


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