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Journal of Virology, April 2005, p. 5232-5237, Vol. 79, No. 8
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.8.5232-5237.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of a New Quaternary Neutralizing Epitope on Human Immunodeficiency Virus Type 1 Virus Particles

Miroslaw K. Gorny,1 Leonidas Stamatatos,2 Barbara Volsky,1 Kathy Revesz,3 Constance Williams,1 Xiao-Hong Wang,3 Sandra Cohen,1 Robert Staudinger,3 and Susan Zolla-Pazner1,3*

Department of Pathology, New York University School of Medicine,1 Research Enhancement Award Program, Veterans Affairs New York Harbor Healthcare System, New York, New York,3 Seattle Biomedical Research Institute, Seattle, Washington2

Received 7 September 2004/ Accepted 1 December 2004

The selection of human monoclonal antibodies (MAbs) specific for human immunodeficiency virus (HIV) type 1 by binding assays may fail to identify Abs to quaternary epitopes on the intact virions. The HIV neutralization assay was used for the selection of human MAb 2909, which potently neutralizes SF162 and recognizes an epitope on the virus surface but not on soluble proteins. Three regions of gp120, the V2 and V3 loops and the CD4 binding domain, contribute to the epitope recognized by MAb 2909. The existence of such a unique MAb, which defines a complex epitope formed by a quaternary structure, suggests that there may be other new neutralizing HIV epitopes to target with vaccines.

* Corresponding author. Mailing address: Veterans Affairs Medical Center, 423 East 23rd St., Room 18124N, New York, NY 10010. Phone: (212) 263-6769. Fax: (212) 951-6321. E-mail: zollas01{at}popmail.med.nyu.edu.

Journal of Virology, April 2005, p. 5232-5237, Vol. 79, No. 8
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.8.5232-5237.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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