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Journal of Virology, April 2005, p. 4952-4964, Vol. 79, No. 8
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.8.4952-4964.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Host and Viral Proteins in the Virion of Kaposi's Sarcoma-Associated Herpesvirus

Jill T. Bechtel,1* Richard C. Winant,2 and Don Ganem1

Department of Microbiology and G. W. Hooper Foundation, Howard Hughes Medical Institute, University of California, San Francisco,1 Protein and Nucleic Acid Facility, Stanford University Medical Center, Palo Alto, California2

Received 15 October 2004/ Accepted 1 December 2004

Infection of cultured cells with Kaposi's sarcoma associated herpesvirus (KSHV) typically establishes a latent infection, in which only a few viral genes are expressed. Recently, it has been reported that a subset of lytic genes are transiently expressed very early after viral entry but that this burst of abortive lytic gene expression is terminated with the supervention of latency (H. H. Krishnan, P. P. Naranatt, M. S. Smith, L. Zeng, C. Bloomer, and B. Chandran, J. Virol. 78:3601-3620, 2004). To identify molecules imported into cells by KSHV that might influence this gene expression program, we have examined the protein composition of the KSHV particle. Immunoblotting of virus particles demonstrated that RTA, the lytic switch protein, and RAP, a viral protein that is a transcriptional and cell cycle modulator, were both incorporated into virus particles. In a second approach, polypeptides isolated from purified virions were identified by mass-spectrometric analysis of their constituent tryptic peptides. With this approach we were able to identify 18 major virion proteins, including structural, regulatory, and signaling proteins of both viral and cellular origin.

* Corresponding author. Mailing address: Department of Microbiology and G. W. Hooper Foundation, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143-0552. Phone: (415) 476-4301. Fax: (415) 476-0939. E-mail: Bechtel{at}itsa.ucsf.edu.

Journal of Virology, April 2005, p. 4952-4964, Vol. 79, No. 8
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.8.4952-4964.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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