This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Greger, J. G.
Right arrow Articles by Skalka, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Greger, J. G.
Right arrow Articles by Skalka, A. M.

 Previous Article  |  Next Article 

Journal of Virology, April 2005, p. 4610-4618, Vol. 79, No. 8
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.8.4610-4618.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Cellular Protein Daxx Interacts with Avian Sarcoma Virus Integrase and Viral DNA To Repress Viral Transcription

James G. Greger,1,2 Richard A. Katz,1 Alexander M. Ishov,3,{dagger} Gerd G. Maul,3 and Anna Marie Skalka1,2*

Fox Chase Cancer Center, Institute for Cancer Research,1 Graduate Group in Cell and Molecular Biology, University of Pennsylvania School of Medicine,2 Wistar Institute, Philadelphia, Pennsylvania3

Received 21 September 2004/ Accepted 19 November 2004

The cellular protein Daxx was identified as an interactor with avian sarcoma virus (ASV) integrase (IN) in a yeast two-hybrid screen. After infection, Daxx-IN interactions were detected by coimmunoprecipitation. An association between Daxx and viral DNA, likely mediated by IN, was also detected by chromatin immunoprecipitation. Daxx was not required for early events in ASV replication, including integration, as Daxx-null cells were transduced as efficiently as Daxx-expressing cells. However, viral reporter gene expression from ASV-based vectors was substantially higher in the Daxx-null cells than in Daxx-complemented cells. Consistent with this observation, histone deacetylases (HDACs) were found to associate with viral DNA in Daxx-complemented cells but not in Daxx-null cells. Furthermore, Daxx protein was induced in an interferon-like manner upon ASV infection. We conclude that Daxx interacts with an IN-viral DNA complex early after infection and may mediate the repression of viral gene expression via the recruitment of HDACs. Our findings provide a novel example of cellular immunity against viral replication in which viral transcription is repressed via the recruitment of antiviral proteins to the viral DNA.

* Corresponding author. Mailing address: Fox Chase Cancer Center, Institute for Cancer Research, 333 Cottman Ave., Philadelphia, PA 19111-2497. Phone: (215) 728-2490. Fax: (215) 728-2778. E-mail: AM_skalka{at}fccc.edu.

{dagger} Present address: University of Florida, Gainesville, FL 32610.

Journal of Virology, April 2005, p. 4610-4618, Vol. 79, No. 8
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.8.4610-4618.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Senigl, F., Plachy, J., Hejnar, J. (2008). The Core Element of a CpG Island Protects Avian Sarcoma and Leukosis Virus-Derived Vectors from Transcriptional Silencing. J. Virol. 82: 7818-7827 [Abstract] [Full Text]  
  • Poleshko, A., Palagin, I., Zhang, R., Boimel, P., Castagna, C., Adams, P. D., Skalka, A. M., Katz, R. A. (2008). Identification of Cellular Proteins That Maintain Retroviral Epigenetic Silencing: Evidence for an Antiviral Response. J. Virol. 82: 2313-2323 [Abstract] [Full Text]  
  • Saffert, R. T., Kalejta, R. F. (2007). Human Cytomegalovirus Gene Expression Is Silenced by Daxx-Mediated Intrinsic Immune Defense in Model Latent Infections Established In Vitro. J. Virol. 81: 9109-9120 [Abstract] [Full Text]  
  • Liu, H.-C. S., Hicks, J. A. (2007). Using Proteomics to Understand Avian Systems Biology and Infectious Disease. Poult. Sci. 86: 1523-1529 [Abstract] [Full Text]  
  • Gaddy, D. F., Lyles, D. S. (2007). Oncolytic Vesicular Stomatitis Virus Induces Apoptosis via Signaling through PKR, Fas, and Daxx. J. Virol. 81: 2792-2804 [Abstract] [Full Text]  
  • Katz, R. A., Jack-Scott, E., Narezkina, A., Palagin, I., Boimel, P., Kulkosky, J., Nicolas, E., Greger, J. G., Skalka, A. M. (2007). High-Frequency Epigenetic Repression and Silencing of Retroviruses Can Be Antagonized by Histone Deacetylase Inhibitors and Transcriptional Activators, but Uniform Reactivation in Cell Clones Is Restricted by Additional Mechanisms. J. Virol. 81: 2592-2604 [Abstract] [Full Text]  
  • Chen, J.-z., Ji, C.-n., Xu, G.-l., Pang, R.-y., Yao, J.-h., Zhu, H.-z., Xue, J.-l., Jia, W. (2006). DAXX interacts with phage {Phi}C31 integrase and inhibits recombination. Nucleic Acids Res 34: 6298-6304 [Abstract] [Full Text]  
  • Cantrell, S. R., Bresnahan, W. A. (2006). Human Cytomegalovirus (HCMV) UL82 Gene Product (pp71) Relieves hDaxx-Mediated Repression of HCMV Replication.. J. Virol. 80: 6188-6191 [Abstract] [Full Text]  
  • Preston, C. M., Nicholl, M. J. (2006). Role of the cellular protein hDaxx in human cytomegalovirus immediate-early gene expression.. J. Gen. Virol. 87: 1113-1121 [Abstract] [Full Text]  
  • Saffert, R. T., Kalejta, R. F. (2006). Inactivating a Cellular Intrinsic Immune Defense Mediated by Daxx Is the Mechanism through Which the Human Cytomegalovirus pp71 Protein Stimulates Viral Immediate-Early Gene Expression.. J. Virol. 80: 3863-3871 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»