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Journal of Virology, April 2005, p. 4540-4544, Vol. 79, No. 7
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.7.4540-4544.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Herpes Simplex Virus Entry Mediator Associates in Infected Cells in a Complex with Viral Proteins gD and at Least gH

Pilar Perez-Romero,1 Aleida Perez,1 Althea Capul,1 Rebecca Montgomery,2 and A. Oveta Fuller3*

Department of Microbiology and Immunology,1 Program in Cellular and Molecular Biology, School of Medicine, University of Michigan, Ann Arbor, Michigan,3 Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin2

Received 18 June 2004/ Accepted 19 October 2004

We examined herpes simplex virus (HSV)-infected human HEp-2 cells or porcine cells that express herpes virus entry mediator (HVEM) for virus and receptor protein interactions. Antibody to HVEM, or its viral ligand gD, coimmunoprecipitated several similar proteins. A prominent 110-kDa protein that coprecipitated was identified as gH. The HVEM/gD/gH complex was detected with mild or stringent cell lysis conditions. It did not form in cells infected with HSV-1(KOS)Rid1 virus or with null virus lacking gD, gH, or gL. Thus, in cells a complex forms through physical associations of HVEM, gD, and at least gH.

* Corresponding author. Mailing address: 6736 Medical Sciences II, University of Michigan, Ann Arbor, MI 48109-0620. Phone: (734) 647-3830. Fax: (734) 764-3562. E-mail: fullerao{at}umich.edu.

Journal of Virology, April 2005, p. 4540-4544, Vol. 79, No. 7
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.7.4540-4544.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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