This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coskun, A. K.
Right arrow Articles by Sutton, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coskun, A. K.
Right arrow Articles by Sutton, R. E.

 Previous Article  |  Next Article 

Journal of Virology, April 2005, p. 4150-4158, Vol. 79, No. 7
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.7.4150-4158.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Expression of Glucose Transporter 1 Confers Susceptibility to Human T-Cell Leukemia Virus Envelope-Mediated Fusion

Ayse Kubra Coskun1 and Richard E. Sutton1,2*

Department of Molecular Virology and Microbiology,1 Center for Cell and Gene Therapy and Department of Medicine, Division of Infectious Diseases, Baylor College of Medicine, Houston, Texas2

Received 9 August 2004/ Accepted 12 November 2004

Human T-cell leukemia virus type 1 (HTLV-1) was the first human retrovirus identified and causes both adult T-cell leukemia/lymphoma and tropical spastic paraparesis/HTLV-1-associated myelopathy, among other disorders. In vitro, HTLV-1 has an extremely broad host cell tropism in that it is capable of infecting most mammalian cell types, although at the same time viral titers remain relatively low. Despite years of study, only recently has a bona fide candidate cellular receptor, glucose transporter 1 (glut-1), been identified. Although glut-1 was shown to bind specifically to the ectodomain of HTLV-1 and HTLV-2 envelope glycoproteins, which was reversible with small interfering RNA directed against glut-1, cellular susceptibility to HTLV upon expression of glut-1 was not established. Here we show that expression of glut-1 in relatively resistant MDBK cells conferred increased susceptibility to both HTLV-1- and HTLV-2-pseudotyped particles. glut-1 also markedly increased syncytium formation in MDBK cells after exposure to HTLV-1. Another assay also demonstrated HTLV-1 envelope-cell fusion in the presence of glut-1. Taken together, these results provide additional evidence that glut-1 is a receptor for HTLV.

* Corresponding author. Mailing address: Department of Molecular Virology and Microbiology, Baylor College of Medicine, Room 917D, One Baylor Plaza, Houston, TX 77030. Phone: (713) 798-4096. Fax: (713) 798-3586. E-mail: rsutton{at}bcm.tmc.edu.

Journal of Virology, April 2005, p. 4150-4158, Vol. 79, No. 7
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.7.4150-4158.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Takenouchi, N., Jones, K. S., Lisinski, I., Fugo, K., Yao, K., Cushman, S. W., Ruscetti, F. W., Jacobson, S. (2007). GLUT1 Is Not the Primary Binding Receptor but Is Associated with Cell-to-Cell Transmission of Human T-Cell Leukemia Virus Type 1. J. Virol. 81: 1506-1510 [Abstract] [Full Text]  
  • Plancoulaine, S., Gessain, A., Tortevoye, P., Boland-Auge, A., Vasilescu, A., Matsuda, F., Abel, L. (2006). A major susceptibility locus for HTLV-1 infection in childhood maps to chromosome 6q27. Hum Mol Genet 15: 3306-3312 [Abstract] [Full Text]  
  • Jones, K. S., Fugo, K., Petrow-Sadowski, C., Huang, Y., Bertolette, D. C., Lisinski, I., Cushman, S. W., Jacobson, S., Ruscetti, F. W. (2006). Human T-Cell Leukemia Virus Type 1 (HTLV-1) and HTLV-2 Use Different Receptor Complexes To Enter T Cells.. J. Virol. 80: 8291-8302 [Abstract] [Full Text]  
  • Ghez, D., Lepelletier, Y., Lambert, S., Fourneau, J.-M., Blot, V., Janvier, S., Arnulf, B., van Endert, P. M., Heveker, N., Pique, C., Hermine, O. (2006). Neuropilin-1 Is Involved in Human T-Cell Lymphotropic Virus Type 1 Entry. J. Virol. 80: 6844-6854 [Abstract] [Full Text]  
  • Si, Z., Vandegraaff, N., O'hUigin, C., Song, B., Yuan, W., Xu, C., Perron, M., Li, X., Marasco, W. A., Engelman, A., Dean, M., Sodroski, J. (2006). Evolution of a cytoplasmic tripartite motif (TRIM) protein in cows that restricts retroviral infection. Proc. Natl. Acad. Sci. USA 103: 7454-7459 [Abstract] [Full Text]  
  • Coskun, A. K., van Maanen, M., Nguyen, V., Sutton, R. E. (2006). Human chromosome 2 carries a gene required for production of infectious human immunodeficiency virus type 1.. J. Virol. 80: 3406-3415 [Abstract] [Full Text]  
  • Xie, L., Green, P. L. (2005). Envelope Is a Major Viral Determinant of the Distinct In Vitro Cellular Transformation Tropism of Human T-Cell Leukemia Virus Type 1 (HTLV-1) and HTLV-2. J. Virol. 79: 14536-14545 [Abstract] [Full Text]  
  • Jones, K. S., Petrow-Sadowski, C., Bertolette, D. C., Huang, Y., Ruscetti, F. W. (2005). Heparan Sulfate Proteoglycans Mediate Attachment and Entry of Human T-Cell Leukemia Virus Type 1 Virions into CD4+ T Cells. J. Virol. 79: 12692-12702 [Abstract] [Full Text]  
  • Manel, N., Battini, J.-L., Sitbon, M. (2005). Human T Cell Leukemia Virus Envelope Binding and Virus Entry Are Mediated by Distinct Domains of the Glucose Transporter GLUT1. J. Biol. Chem. 280: 29025-29029 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»