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Journal of Virology, March 2005, p. 3883-3887, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3883-3887.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Novel Polyomavirus Detected in the Feces of a Chimpanzee by Nested Broad-Spectrum PCR

Reimar Johne,1* Dirk Enderlein,1 Hermann Nieper,2 and Hermann Müller1

Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig,1 Landesuntersuchungsanstalt für das Gesundheits und Veterinärwesen Sachsen, Leipzig, Germany2

Received 12 October 2004/ Accepted 2 November 2004

In order to screen for new polyomaviruses in samples derived from various animal species, degenerated PCR primer pairs were constructed. By using a nested PCR protocol, the sensitive detection of nine different polyomavirus genomes was demonstrated. The screening of field samples revealed the presence of a new polyomavirus, tentatively designated chimpanzee polyomavirus (ChPyV), in the feces of a juvenile chimpanzee (Pan troglodytes). Analysis of the region encoding the major capsid protein VP1 revealed a unique insertion in the EF loop of the protein and showed that ChPyV is a distinct virus related to the monkey polyomavirus B-lymphotropic polyomavirus and the human polyomavirus JC polyomavirus.

* Corresponding author. Mailing address: Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, D-04103 Leipzig, Germany. Phone: 49(0)341-9738204. Fax: 49(0)341-9738219. E-mail: johne{at}vetmed.uni-leipzig.de.

Journal of Virology, March 2005, p. 3883-3887, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3883-3887.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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