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Journal of Virology, March 2005, p. 3878-3882, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3878-3882.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Splicing of Rhesus Rhadinovirus R15 and ORF74 Bicistronic Transcripts during Lytic Infection and Analysis of Effects on Production of vCD200 and vGPCR

Carly L. Pratt,1,2,{dagger} Ryan D. Estep,1,2,{dagger} and Scott W. Wong1,2,3*

Vaccine and Gene Therapy Institute, Oregon Health and Science University, West Campus,1 Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton,3 Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon2

Received 30 January 2004/ Accepted 1 November 2004

Rhesus macaque rhadinovirus (RRV) is the rhesus macaque homologue of human herpesvirus 8 (HHV-8). Here we examine expression of RRV R15 and ORF74, homologues of K14 and ORF74 of HHV-8, respectively. As in HHV-8, transcripts encoding RRV R15 and ORF74 are bicistronic. However, unlike what has been suggested for HHV-8, RRV R15- and ORF74-encoding transcripts are expressed late during lytic infection and undergo unique splicing events that result in the production of transcripts capable of encoding vGPCR, as well as membrane-associated and secreted forms of vCD200. The alternative splicing for vCD200 has implications for viral pathogenesis.

* Corresponding author. Mailing address: Vaccine and Gene Therapy Institute, Oregon Health and Science University, West Campus, 505 N.W. 185th Ave., Beaverton, OR 97006. Phone: (503) 690-5285. Fax: (503) 418-2719. E-mail: wongs{at}ohsu.edu.

{dagger} C.L.P. and R.D.E. contributed equally to the study.

Journal of Virology, March 2005, p. 3878-3882, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3878-3882.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Langlais, C. L., Jones, J. M., Estep, R. D., Wong, S. W. (2006). Rhesus Rhadinovirus R15 Encodes a Functional Homologue of Human CD200. J. Virol. 80: 3098-3103 [Abstract] [Full Text]  


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