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Journal of Virology, March 2005, p. 3841-3845, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3841-3845.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Deletion of the Second Immunoglobulin-Like Domain of Nectin-1 Alters Its Intracellular Processing and Localization and Ability To Mediate Entry of Herpes Simplex Virus

Frank Struyf, Aileen E. Plate, and Patricia G. Spear*

Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois

Received 13 August 2004/ Accepted 26 October 2004

Nectin-1 is an immunoglobulin (Ig)-like entry receptor for herpes simplex virus (HSV). Like other nectins, nectin-1 forms dimers and mediates cell adhesion through interactions with other nectins. We constructed a second-domain deletion mutant of nectin-1 (nectin-1-{Delta}2) to examine the role of the second Ig-like domain in HSV entry. Nectin-1-{Delta}2 exhibited a severely reduced ability to mediate HSV entry and accumulated in the endoplasmic reticulum but retained the ability to interact with its HSV ligand, gD. The failure of nectin-1-{Delta}2 to mediate HSV entry probably resulted from its failure to be transported to a membrane targeted by HSV for viral entry.

* Corresponding author. Mailing address: Northwestern University, The Feinberg School of Medicine, Department of Microbiology and Immunology, Searle 6-447, 320 E. Superior St., Chicago, IL 60611. Phone: (312) 503-1343. Fax: (312) 503-1339. E-mail: p-spear{at}northwestern.edu.

Journal of Virology, March 2005, p. 3841-3845, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3841-3845.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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  • Ono, E., Tomioka, Y., Watanabe, Y., Amagai, K., Morimatsu, M., Shinya, K., Cherel, P. (2007). Comparison of the antiviral potentials among the pseudorabies-resistant transgenes encoding different soluble forms of porcine nectin-1 in transgenic mice. J. Gen. Virol. 88: 2636-2641 [Abstract] [Full Text]  


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