This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, C.-W.
Right arrow Articles by Kim, J.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, C.-W.
Right arrow Articles by Kim, J.-H.
Right arrowPubmed/NCBI databases
*Protein
*Substance via MeSH
Medline Plus Health Information
*Bird Flu

 Previous Article  |  Next Article 

Journal of Virology, March 2005, p. 3692-3702, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3692-3702.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of Highly Pathogenic H5N1 Avian Influenza A Viruses Isolated from South Korea

Chang-Won Lee,1 David L. Suarez,1 Terrence M. Tumpey,2 Haan-Woo Sung,3 Yong-Kuk Kwon,3 Youn-Jeong Lee,3 Jun-Gu Choi,3 Seong-Joon Joh,3 Min-Chul Kim,3 Eun-Kyoung Lee,3 Jong-Myung Park,3 Xiuhua Lu,2 Jacqueline M. Katz,2 Erica Spackman,1 David E. Swayne,1* and Jae-Hong Kim3

Southeast Poultry Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Athens,1 Centers for Disease Control and Prevention, Atlanta, Georgia,2 National Veterinary Research and Quarantine Service, Anyang, Korea3

Received 4 August 2004/ Accepted 13 October 2004

An unprecedented outbreak of H5N1 highly pathogenic avian influenza (HPAI) has been reported for poultry in eight different Asian countries, including South Korea, since December 2003. A phylogenetic analysis of the eight viral genes showed that the H5N1 poultry isolates from South Korea were of avian origin and contained the hemagglutinin and neuraminidase genes of the A/goose/Guangdong/1/96 (Gs/Gd) lineage. The current H5N1 strains in Asia, including the Korean isolates, share a gene constellation similar to that of the Penfold Park, Hong Kong, isolates from late 2002 and contain some molecular markers that seem to have been fixed in the Gs/Gd lineage virus since 2001. However, despite genetic similarities among recent H5N1 isolates, the topology of the phylogenetic tree clearly differentiates the Korean isolates from the Vietnamese and Thai isolates which have been reported to infect humans. A representative Korean isolate was inoculated into mice, with no mortality and no virus being isolated from the brain, although high titers of virus were observed in the lungs. The same isolate, however, caused systemic infections in chickens and quail and killed all of the birds within 2 and 4 days of intranasal inoculation, respectively. This isolate also replicated in multiple organs and tissues of ducks and caused some mortality. However, lower virus titers were observed in all corresponding tissues of ducks than in chicken and quail tissues, and the histological lesions were restricted to the respiratory tract. This study characterizes the molecular and biological properties of the H5N1 HPAI viruses from South Korea and emphasizes the need for comparative analyses of the H5N1 isolates from different countries to help elucidate the risk of a human pandemic from the strains of H5N1 HPAI currently circulating in Asia.

* Corresponding author. Mailing address: Southeast Poultry Research Laboratory, USDA-ARS, 934 College Station Rd., Athens, GA 30605. Phone: (706) 546-3433. Fax: (706) 546-3161. E-mail: dswayne{at}seprl.usda.gov.

Journal of Virology, March 2005, p. 3692-3702, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3692-3702.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Das, A., Spackman, E., Pantin-Jackwood, M. J., Suarez, D. L. (2009). Removal of real-time reverse transcription polymerase chain reaction (RT-PCR) inhibitors associated with cloacal swab samples and tissues for improved diagnosis of Avian influenza virus by RT-PCR. jvdi 21: 771-778 [Abstract] [Full Text]  
  • Lycett, S. J., Ward, M. J., Lewis, F. I., Poon, A. F. Y., Kosakovsky Pond, S. L., Brown, A. J. L. (2009). Detection of Mammalian Virulence Determinants in Highly Pathogenic Avian Influenza H5N1 Viruses: Multivariate Analysis of Published Data. J. Virol. 83: 9901-9910 [Abstract] [Full Text]  
  • Ma, Y., Feng, Y., Liu, D., Gao, G. F. (2009). Avian influenza virus, Streptococcus suis serotype 2, severe acute respiratory syndrome-coronavirus and beyond: molecular epidemiology, ecology and the situation in China. Phil Trans R Soc B 364: 2725-2737 [Abstract] [Full Text]  
  • Tsukamoto, K., Ashizawa, H., Nakanishi, K., Kaji, N., Suzuki, K., Okamatsu, M., Yamaguchi, S., Mase, M. (2008). Subtyping of Avian Influenza Viruses H1 to H15 on the Basis of Hemagglutinin Genes by PCR Assay and Molecular Determination of Pathogenic Potential. J. Clin. Microbiol. 46: 3048-3055 [Abstract] [Full Text]  
  • Wang, J., Vijaykrishna, D., Duan, L., Bahl, J., Zhang, J. X., Webster, R. G., Peiris, J. S. M., Chen, H., Smith, G. J. D., Guan, Y. (2008). Identification of the Progenitors of Indonesian and Vietnamese Avian Influenza A (H5N1) Viruses from Southern China. J. Virol. 82: 3405-3414 [Abstract] [Full Text]  
  • Sharkey, K. J, Bowers, R. G, Morgan, K. L, Robinson, S. E, Christley, R. M (2008). Epidemiological consequences of an incursion of highly pathogenic H5N1 avian influenza into the British poultry flock. Proc R Soc B 275: 19-28 [Abstract] [Full Text]  
  • van Riel, D., Munster, V. J., de Wit, E., Rimmelzwaan, G. F., Fouchier, R. A.M., Osterhaus, A. D.M.E., Kuiken, T. (2007). Human and Avian Influenza Viruses Target Different Cells in the Lower Respiratory Tract of Humans and Other Mammals. Am. J. Pathol. 171: 1215-1223 [Abstract] [Full Text]  
  • Rinder, M., Lang, V., Fuchs, C., Hafner-Marx, A., Bogner, K.-H., Neubauer, A., Buttner, M., Rinder, H. (2007). Genetic Evidence for Multi-event Imports of Avian Influenza Virus a (H5N1) into Bavaria, Germany. jvdi 19: 279-282 [Abstract] [Full Text]  
  • Ge, J., Deng, G., Wen, Z., Tian, G., Wang, Y., Shi, J., Wang, X., Li, Y., Hu, S., Jiang, Y., Yang, C., Yu, K., Bu, Z., Chen, H. (2007). Newcastle Disease Virus-Based Live Attenuated Vaccine Completely Protects Chickens and Mice from Lethal Challenge of Homologous and Heterologous H5N1 Avian Influenza Viruses. J. Virol. 81: 150-158 [Abstract] [Full Text]  
  • Zhou, J.-Y., Shen, H.-G., Chen, H.-X., Tong, G.-Z., Liao, M., Yang, H.-C., Liu, J.-X. (2006). Characterization of a highly pathogenic H5N1 influenza virus derived from bar-headed geese in China. J. Gen. Virol. 87: 1823-1833 [Abstract] [Full Text]  
  • van Riel, D., Munster, V. J., de Wit, E., Rimmelzwaan, G. F., Fouchier, R. A. M., Osterhaus, A. D. M. E., Kuiken, T. (2006). H5N1 Virus Attachment to Lower Respiratory Tract. Science 312: 399-399 [Abstract] [Full Text]  
  • Chen, H., Smith, G. J. D., Li, K. S., Wang, J., Fan, X. H., Rayner, J. M., Vijaykrishna, D., Zhang, J. X., Zhang, L. J., Guo, C. T., Cheung, C. L., Xu, K. M., Duan, L., Huang, K., Qin, K., Leung, Y. H. C., Wu, W. L., Lu, H. R., Chen, Y., Xia, N. S., Naipospos, T. S. P., Yuen, K. Y., Hassan, S. S., Bahri, S., Nguyen, T. D., Webster, R. G., Peiris, J. S. M., Guan, Y. (2006). Establishment of multiple sublineages of H5N1 influenza virus in Asia: Implications for pandemic control. Proc. Natl. Acad. Sci. USA 103: 2845-2850 [Abstract] [Full Text]  
  • Maines, T. R., Lu, X. H., Erb, S. M., Edwards, L., Guarner, J., Greer, P. W., Nguyen, D. C., Szretter, K. J., Chen, L.-M., Thawatsupha, P., Chittaganpitch, M., Waicharoen, S., Nguyen, D. T., Nguyen, T., Nguyen, H. H. T., Kim, J.-H., Hoang, L. T., Kang, C., Phuong, L. S., Lim, W., Zaki, S., Donis, R. O., Cox, N. J., Katz, J. M., Tumpey, T. M. (2005). Avian Influenza (H5N1) Viruses Isolated from Humans in Asia in 2004 Exhibit Increased Virulence in Mammals. J. Virol. 79: 11788-11800 [Abstract] [Full Text]  
  • Kwon, Y-K., Joh, S-J., Kim, M-C., Lee, Y-J., Choi, J-G., Lee, E-K., Wee, S-H., Sung, H-W., Kwon, J-H., Kang, M-I., Kim, J-H. (2005). Highly Pathogenic Avian Influenza in Magpies (Pica pica sericea) in South Korea. J Wildl Dis 41: 618-623 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»